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Strain Detail Sheet

Published: 06/16/2019
Revised: 04/27/2020

Strain Name:
B6.KSB-fe/Mmmh
Stock Number:
000186-MU
Citation ID:
RRID:MMRRC_000186-MU
Major Collection:
Mammalian Pigmentation

Strain Information

Gene Details

fe
Name: faded; faded
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Spontaneous Mutation
Genotype Determination

Strain Description

Phenotype
The locus is not defined. However it is likely that faded is an allele of London grey (MMRRC:000185). Faded mice and London grey mice both are characterized by reduced pigmentation and white based hairs. Faded shows the more extreme phenotype.

Faded is recessive to wild type. C57BL/6-fe/fe mice are a uniform pale gray, growing more pale with succeeding molts. Blowing back the hairs reveals that they have with maturity developed an increasingly pronounced white-based phenotype, meaning that the bases of the hairs are less pigmented than is normal. The phenotype is similar to but much more pronounced compared with that seen in London grey mice. Lamoreux has mated London grey with faded mice. The unpublished results suggest that the two are allelic and that London grey is dominant to faded. Additional unpublished results suggest that faded is not located (as published) near the Mitf locus on chromosome 6. The similarities in phenotype and the possibility of allelism suggest that the faded defect may lie in the transfer of melanosomes from the pigment cell into the hair, or may be the result of progressive loss of pigment cells during growth of the hair.
Mammalian Phenotype Terms
Allelic Composition: fe/fe (Genetic Background: KSB )
MeSH Terms
  • Aging
  • Animals
  • Genes, Recessive
  • Hair Color
  • Mice
  • Mice, Inbred Strains/genetics
  • Mutation
  • Pigmentation
  • Chromosome Mapping
  • Chromosomes
  • Genetic Linkage
  • Hair Color/genetics
  • Mice, Mutant Strains/genetics
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Pigmentation
  • Cell Biology of Organelles
  • Developmental Biology

Strain Origin

Submitter
Dr. Lynn Lamoreux, Texas A&M University
Primary Reference
  • Oh YS, Tomita T, Kondo K. Faded, a mutation in the KSB strain of mouse which shows age-related pigment changes. Jikken Dobutsu. 1986 Apr;35(2):131-8. (Medline PMID: 3732403)
  • Oh YS, Tomita T. Linkage of faded gene (fe) to chromosome 6 of the mouse. Jikken Dobutsu. 1987 Jan;36(1):73-7. (Medline PMID: 3816991)
Strain Development
This mutation was reported by Professor YS Oh, working with Professor T. Tomita at Nagoya University in Japan. Professors Oh and Tomita transferred the mutation to Lamoreux, who backcrossed to C57BL/6J for more than 20 generations and then maintained it by sib mating.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Special Considerations

Feed 9% fat breeder diet

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Breeding

MMRRC Breeding System
Sib Mating
Breeding Scheme(s)
Homozygous female x Homozygous male
Generation
N>10F>5 As of: 11/12/2004
Overall Breeding Performance
Good

Reproductive Statistics

Average litter size
7
Recommended wean age
3 to 4 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
000186-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit 		Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000186-MU-SPERM Cryo-preserved spermatozoa $546.25 / $1,021.25
Non-Profit / For-Profit 		Aliquot Approximate quantity3
000186-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit 		Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.