Strain Name:
B6;D2-Tg(Camk2a-cre)1Lfr/Mmucd
Stock Number:
000189-UCD
Citation ID:
RRID:MMRRC_000189-UCD

Strain Information

cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Tg(Camk2a-cre)1Lfr
Name: transgene insertion 1, Louis F Reichardt
Synonyms: CamKIIcre, CaMKcre, CaMKII-cre
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genetic Alterations
Transgenic insertion of CamKII-cre cassette into unknown region of genome.
Genotype Determination
Phenotype
No distinct phenotype.
MeSH Terms
  • Animals
  • Cell Count
  • DNA-Binding Proteins/metabolism
  • Dendrites/metabolism
  • Dendrites/pathology
  • Mice
  • Mice, Transgenic
  • Mutation/genetics
  • Neocortex/metabolism
  • Neocortex/pathology
  • Nerve Growth Factors/metabolism
  • Neurodegenerative Diseases/metabolism
  • Neurodegenerative Diseases/pathology
  • Neurons/metabolism
  • Neurons/pathology
  • Pyramidal Cells/metabolism
  • Receptor, trkB/genetics
  • Receptor, trkB/metabolism
  • Transcription Factors/metabolism
  • beta-Galactosidase/metabolism
  • Antigens, Differentiation/metabolism
  • Axons/metabolism
  • Brain-Derived Neurotrophic Factor/metabolism
  • Calcium-Calmodulin-Dependent Protein Kinase Type 2
  • Calcium-Calmodulin-Dependent Protein Kinases/genetics
  • Hippocampus/cytology
  • Hippocampus/metabolism
  • In Vitro Techniques
  • Long-Term Potentiation/genetics
  • Mice, Inbred C57BL
  • Neuronal Plasticity/genetics
  • Patch-Clamp Techniques
  • Presynaptic Terminals/metabolism
  • RNA, Messenger/biosynthesis
  • Receptor, Nerve Growth Factor/antagonists & inhibitors
  • Receptor, Nerve Growth Factor/metabolism
  • Receptor, trkB/deficiency
  • Receptors, Glutamate/metabolism
  • Signal Transduction/genetics
  • Stem Cells
Strain Development
fBZ heterozygous mice were bred with CaMKcre transgenic mice to generate mice heterozygous for both the fBZ allele and the CaMKcre transgene (fBZ/+;CaMKcre/+). The double heterozygous mice were mated with fBZ heterozygotes (fBZ/+;+/+) to obtain trkB conditional knockouts (fBZ/fBZ;CaMKcre/+) and their control animals. Animals heterozygous for both fBZ and CaMKcre were also used to analyze the pattern of Cre-mediated trkB knockout in the brain by using X-gal staining and b-galactosidase immunohistochemistry. The trkBlacZ allele was generated by crossing a cre transgene under the control of the b-actin promoter into fBZ/1 mice. Genotypes of mice were determined by PCR or Southern hybridization. Since transfer to MMRRC this strain has been further backcrossed to C57BL/6J to produce the congenic strain, B6.Cg-Tg(Camk2a-cre)1Lfr/Mmcd (see MMRRC #15962), that is 99% C57BL/6J by micro satellite testing.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Louis Reichardt, Ph.D.,  University of California, San Francisco
Primary Reference
  • Xu B, Zang K, Ruff NL, Zhang YA, McConnell SK, Stryker MP, Reichardt LF. Cortical degeneration in the absence of neurotrophin signaling: dendritic retraction and neuronal loss after removal of the receptor TrkB. Neuron. 2000 Apr;26(1):233-45. (Medline PMID: 10798407)
  • Xu B, Gottschalk W, Chow A, Wilson RI, Schnell E, Zang K, Wang D, Nicoll RA, Lu B, Reichardt LF. The role of brain-derived neurotrophic factor receptors in the mature hippocampus: modulation of long-term potentiation through a presynaptic mechanism involving TrkB. J Neurosci. 2000 Sep 15;20(18):6888-97. (Medline PMID: 10995833)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Sibling mating, Backcross
Breeding Scheme(s)
  • Hemizygous female x Sibling wildtype male
  • Sibling wildtype female x Hemizygous male
  • C57BL/6 female x Hemizygous male
  • Hemizygous female x C57BL/6 male
Generation
approximately N7 to C57BL/6
Overall Breeding Performance
Good
Average litter size
10
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000189-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000189-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
000189-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.