Strain Detail Sheet

Strain Name    :

B6;D2-Tg(Camk2a-cre)1Lfr/Mmucd

Stock Number :

000189-UCD

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(Camk2a-cre)1Lfr
Name: transgene insertion 1, Louis F Reichardt
Alteration at locus: Transgenic (cre)
Transgene: cre (Bacteriophage P1)
Name: cre recombinase
Alteration at locus: Transgenic (cre)
Promoter: Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Alteration at locus: Transgenic (cre)

Genetic Alterations:
Transgenic insertion of CamKII-cre cassette into unknown region of genome.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

No distinct phenotype.

Strain of Origin: B6D2F1

Strain genetic background: C57BL/6

Strain Development: fBZ heterozygous mice were bred with CaMKcre transgenic mice to generate mice heterozygous for both the fBZ allele and the CaMKcre transgene (fBZ/+;CaMKcre/+). The double heterozygous mice were mated with fBZ heterozygotes (fBZ/+;+/+) to obtain trkB conditional knockouts (fBZ/fBZ;CaMKcre/+) and their control animals. Animals heterozygous for both fBZ and CaMKcre were also used to analyze the pattern of Cre-mediated trkB knockout in the brain by using X-gal staining and b-galactosidase immunohistochemistry. The trkBlacZ allele was generated by crossing a cre transgene under the control of the b-actin promoter into fBZ/1 mice. Genotypes of mice were determined by PCR or Southern hybridization. Since transfer to MMRRC this strain has been further backcrossed to C57BL/6J to produce the congenic strain, B6.Cg-Tg(Camk2a-cre)1Lfr/Mmcd (MMRRC #15962), that is 99% C57BL/6J by micro satellite testing.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Louis Reichardt, Ph.D., University of California - San Francisco

Primary Reference:

  • Xu B, Zang K, Ruff NL, Zhang YA, McConnell SK, Stryker MP, Reichardt LF. Cortical degeneration in the absence of neurotrophin signaling: dendritic retraction and neuronal loss after removal of the receptor TrkB. Neuron. 2000 Apr;26(1):233-45. (Medline PMID: 10798407)
  • Xu B, Gottschalk W, Chow A, Wilson RI, Schnell E, Zang K, Wang D, Nicoll RA, Lu B, Reichardt LF. The role of brain-derived neurotrophic factor receptors in the mature hippocampus: modulation of long-term potentiation through a presynaptic mechanism involving TrkB. J Neurosci. 2000 Sep 15;20(18):6888-97. (Medline PMID: 10995833)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Sibling mating, Backcross

Breeding Scheme(s):

  • Hemizygous female x Sibling wildtype male
  • Sibling wildtype female x Hemizygous male
  • C57BL/6 female x Hemizygous male
  • Hemizygous female x C57BL/6 male

Generation: approximately N7 to C57BL/6

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: similar to C57BL/6 similar to C57BL/6

Average litter size: 10

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000189-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000189-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
000189-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/23/2014