Strain Detail Sheet

Strain Name    :

STOCK Bdnftm1Lfr/Mmucd

Stock Number :

000190-UCD

Other Names   :

BDNFneo, STOCK Bdnftm1Lfr/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Bdnftm1Lfr
Name: targeted mutation 1, Louis F Reichardt
Chromosome: 2; 62.0 cM
Alteration at locus: Targeted Mutation
Gene Symbol: Bdnf
Name: brain derived neurotrophic factor
Chromosome: 2
Alteration at locus: Targeted Mutation

Genetic Alterations:
neo cassette inserted into coding exon for mature BDNF

Genotype Determination:

ES Cell Line: D3 derived from B6;129S2

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Extensive loss of placodal sensory neurons, cardiac deficit.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Bdnftm1Lfr/Bdnf+
        involves: 129S2/SvPas * C57BL/6J
  • growth/size phenotype
    • obese (MGI Ref ID J:84683)
      • an 85% increase in body mass is seen at 8 weeks of age
Bdnftm1Lfr/Bdnftm1Lfr
        involves: 129S2/SvPas
  • digestive/alimentary phenotype
    • abnormal circumvallate papillae (MGI Ref ID J:55127)
      • the circumvallate papilla is reduced by 39% compared to wild-type littermates and lack morphological integrity
    • abnormal fungiform papillae (MGI Ref ID J:55127)
      • a 57% reduction in the number of fungiform papillae is seen with the remaining papillae concentrated on the anterior-most portion of the tongue
      • papillae width is reduced by 33% and the size of the taste buds reduced by 18%
    • decreased tongue size (MGI Ref ID J:55127)
      • tongue length averages 8.6 mm compared to 10.3 mm in wild-type littermates
  • taste/olfaction phenotype
    • abnormal gustatory papillae taste bud morphology (MGI Ref ID J:55127)
      • the number of circumvallate taste buds is reduced by about 73% compared to wild-type littermates
      • the size of taste buds on the tips of fungiform papillae is reduced by 18%
  • growth/size phenotype
    • decreased body size (MGI Ref ID J:55127)
  • craniofacial phenotype
    • abnormal circumvallate papillae (MGI Ref ID J:55127)
      • the circumvallate papilla is reduced by 39% compared to wild-type littermates and lack morphological integrity
    • abnormal fungiform papillae (MGI Ref ID J:55127)
      • a 57% reduction in the number of fungiform papillae is seen with the remaining papillae concentrated on the anterior-most portion of the tongue
      • papillae width is reduced by 33% and the size of the taste buds reduced by 18%
    • decreased tongue size (MGI Ref ID J:55127)
      • tongue length averages 8.6 mm compared to 10.3 mm in wild-type littermates
    • shortened head (MGI Ref ID J:55127)
      • head length averages 13.7 mm compared to 16.1 mm in wild-type littermates
Bdnftm1Lfr/Bdnftm1Lfr
        involves: 129S2/SvPas * C57BL/6
  • mortality/aging
    • complete postnatal lethality (MGI Ref ID J:67231)
      • most die within 48 hours of birth with a few surviving up to 25 days of age
  • nervous system phenotype
    • abnormal cerebellum external granule cell layer morphology (MGI Ref ID J:67231)
      • an external granule cell layer is present at postnatal day (P) 17 but not at P21 indicating delayed granule cell migration
    • abnormal cranial ganglia morphology (MGI Ref ID J:67231)
      • abnormal vestibular ganglion morphology (MGI Ref ID J:67231)
        • the size of the vestibular ganglion is reduced
      • small geniculate ganglion (MGI Ref ID J:67231)
      • small nodose ganglion (MGI Ref ID J:67231)
      • small petrosal ganglion (MGI Ref ID J:67231)
    • abnormal neuron differentiation (MGI Ref ID J:67231)
      • reduction in neuropeptide and calcium-binding protein expression in specific regions of the brain (cerebral cortex and hippocampus) suggests impaired neuronal differentiation
    • abnormal somatic sensory system morphology (MGI Ref ID J:67231)
      • abnormal sensory ganglion morphology (MGI Ref ID J:67231)
        • virtually all sensory ganglia have deficits in size or cell number
        • small dorsal root ganglion (MGI Ref ID J:67231)
        • small geniculate ganglion (MGI Ref ID J:67231)
        • small nodose ganglion (MGI Ref ID J:67231)
      • decreased sensory neuron number (MGI Ref ID J:67231)
        • sensory neuron number is reduced in the trigeminal ganglion, petrosal-nodose ganglion, mesencephalic trigeminal nucleus, and L4 dorsal root ganglion by 27%, 43%, 39%, and 34%, respectively; however motor neuron numbers appear normal
    • small mesencephalic trigeminal nucleus (MGI Ref ID J:67231)
  • behavior/neurological phenotype
    • abnormal motor capabilities/coordination/movement (MGI Ref ID J:67231)
      • progressively more severe motor defects develop with age
      • abnormal locomotor activation (MGI Ref ID J:67231)
        • homozygotes that survive more than 48 hours display periods of hyperactivity followed by inactivity
      • ataxia (MGI Ref ID J:67231)
      • hunched posture (MGI Ref ID J:67231)
      • impaired righting response (MGI Ref ID J:67231)
      • spinning (MGI Ref ID J:67231)
  • respiratory system phenotype
    • decreased pulmonary respiratory rate (MGI Ref ID J:67231)
      • reduced and irregular breathing develops with age

Strain of Origin: 129/C57BL6

Strain genetic background: ICR

Strain Development: Positive recombinant D3 ES cell clones were injected into C57BL/6 blastocysts. Subsequent KO positive mice were maintained on ICR stock.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Neurobiology

Strain Origin

Donor: Louis F. Reichardt, Ph.D., University of California - San Francisco

Primary Reference:

Jones KR, Farinas I, Backus C, Reichardt LF. Targeted disruption of the BDNF gene perturbs brain and sensory neuron development but not motor neuron development. Cell. 1994 Mar 25;76(6):989-99. (Medline PMID: 8137432)

Colony and Husbandry Information

Special Considerations

Mature onset obesity at 6 months of age.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: albino

Other:

Breeding

MMRRC Breeding System: Inbreeding

Breeding Scheme(s):

  • Heterozygous female x Wildtype male
  • Wildtype female x Heterozygous male

Generation: F2

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 32 weeks 32 weeks

Average litter size: 14

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000190-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000190-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
000190-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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