Strain Detail Sheet

Strain Name    :

STOCK Ntf3tm1Lfr/Mmucd

Stock Number :

000191-UCD

Other Names   :

NT3lacZ-neo, STOCK Ntf3tm1Lfr/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Ntf3tm1Lfr
Name: targeted mutation 1, Louis F Reichardt
Chromosome: 6; 61.0 cM
Alteration at locus: Targeted Mutation
Gene Symbol: Ntf3
Name: neurotrophin 3
Chromosome: 6
Alteration at locus: Targeted Mutation

Genetic Alterations:
lacZ-neo cassette inserted into coding exon of NT3

Genotype Determination:

ES Cell Line: D3 derived from B6;129S2

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Recessive loss of extensive populations of sensory and sympathetic neurons. Cardiac abnormalities. Early postnatal lethal as homozygote.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Ntf3tm1Lfr/Ntf3tm1Lfr
        involves: 129S2/SvPas
  • mortality/aging
    • partial neonatal lethality (MGI Ref ID J:18984)
      • most die within 24 hours with a few (3 of 300 total) surviving for 7 - 15 days
  • nervous system phenotype
    • abnormal cranial ganglia morphology (MGI Ref ID J:18984)
      • loss of 30% of sensory neurons from the petrosal-nodose ganglia
      • cochlear ganglion hypoplasia (MGI Ref ID J:18984)
        • loss of 85% of sensory neurons
      • small geniculate ganglion (MGI Ref ID J:18984)
        • loss of 25% of sensory neurons
      • small trigeminal ganglion (MGI Ref ID J:18984)
        • loss of 61% of sensory neurons
    • abnormal muscle spindle morphology (MGI Ref ID J:132854)
      • fail to form spindles
      • absent muscle spindles (MGI Ref ID J:18984)
    • abnormal sensory neuron projections (MGI Ref ID J:18984)
      • group Ia afferents appear to be absent
    • decreased motor neuron number (MGI Ref ID J:132854)
      • small diameter fusimotor axons are absent in the ventral roots of lumbar nerves
      • elev ated apoptosis of motor neurons between 13.5 and E15.5
    • small dorsal root ganglion (MGI Ref ID J:18984)
      • loss of 60 - 80% of neurons
      • loss of 78% of sensory neurons from the L5 ganglion
      • loss of 62% of sensory neurons from the C1 ganglion
    • small mesencephalic trigeminal nucleus (MGI Ref ID J:18984)
      • loss of 56% of sensory neurons
    • small superior cervical ganglion (MGI Ref ID J:18984)
      • at P0, about a 50% decrease in cell number is detected without any increase in pyknotic neurons
  • behavior/neurological phenotype
    • abnormal food intake (MGI Ref ID J:18984)
      • no evidence of food intake
    • abnormal limb posture (MGI Ref ID J:18984)
      • abnormal limb posture
    • athetotic walking movements (MGI Ref ID J:18984)
      • seen in rare survivors
    • hypoactivity (MGI Ref ID J:18984)
  • muscle phenotype
    • abnormal muscle spindle morphology (MGI Ref ID J:132854)
      • fail to form spindles
      • absent muscle spindles (MGI Ref ID J:18984)
Ntf3tm1Lfr/Ntf3tm1Lfr
        involves: C57BL/6J
  • nervous system phenotype
    • abnormal sensory neuron innervation pattern (MGI Ref ID J:90280)
      • innervation of the somatosensory prominences is virtually absent

Strain of Origin: 129xC57BL/6

Strain genetic background: ICR

Strain Development: Founder 129 x C57BL/6 mice were crossed to Charles River CD1-ICR mice for 15 generations followed by 12 generations of sib mating.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Dr. Louis Reichardt, University of California San Francisco

Primary Reference:

Farinas I, Jones KR, Backus C, Wang XY, Reichardt LF. Severe sensory and sympathetic deficits in mice lacking neurotrophin-3. Nature. 1994 Jun 23;369(6482):658-61. (Medline PMID: 8208292 )

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color:

Other:

Breeding

MMRRC Breeding System: Sib mating

Breeding Scheme(s):

  • Heterozygous female x Wildtype male
  • Wildtype female x Heterozygous male

Generation: F12+

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 36 weeks 36 weeks

Average litter size: 10

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000191-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000191-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
000191-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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