Strain Name:
B6;129X1-Itga8tm1Lfr/Mmucd
Stock Number:
000193-UCD
Citation ID:
RRID:MMRRC_000193-UCD

Strain Information

Itga8tm1Lfr
Name: integrin alpha 8; targeted mutation 1, Louis F Reichardt
Synonyms: alpha8-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
Itga8
Name: integrin alpha 8
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Knockout
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396
Genetic Alterations
Neo cassette inserted into an integrin alpha 8 exon encoding amino acids 362-424.
Genotype Determination
ES Cell Line
JM-1 derived from 129X1/SvJ
Phenotype
Perinatal lethality resulting from failure to form kidney. Also deficit in hair cells in inner ear.
Mammalian Phenotype Terms
Allelic Composition: Itga8tm1Lfr/Itga8tm1Lfr (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

Allelic Composition: Itga8tm1Lfr/Itga8+ (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

MeSH Terms
  • Alleles
  • Animals
  • Blotting, Western
  • Cell Communication/genetics
  • Cell Line/chemistry
  • Cell Line/physiology
  • DNA-Binding Proteins/genetics
  • Epithelial Cells
  • Female
  • Gene Expression Regulation, Developmental/physiology
  • Integrins/genetics
  • Kidney/cytology
  • Kidney/embryology
  • Kidney/pathology
  • Ligands
  • Male
  • Mesoderm/cytology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Morphogenesis/genetics
  • PAX2 Transcription Factor
  • Phenotype
  • Receptor, Nerve Growth Factor
  • Receptors, Fibronectin/genetics
  • Receptors, Nerve Growth Factor/genetics
  • Stem Cells/chemistry
  • Stem Cells/cytology
  • Stem Cells/physiology
  • Transcription Factors/genetics
  • Ureter/cytology
  • Ureter/embryology
  • Ureter/pathology
Strain Development
JM1 embryonic stem cells (J. J. M. and R. A. P., unpublished data) were grown on mitotically inactivated STO cells and electroporated with 25 mg of linearized targeting vector. After 8–10 days in selective medium (300 mg/ml G418 and 0.2 mM FIAU), colonies were picked, expanded, and screened by polymerase chain reaction and Southern blot analysis and used for injection of C57Bl/6 blastocysts. To obtain germline transmission, chimeric male mice were mated to C57Bl/6 females. Since transfer to MMRRC this strain has been further backcrossed to C57BL/6J to produce the congenic strain, B6.129X1-Itga8tm1Lfr/Mmucd (see MMRRC:015964), that is 99% C57BL/6J by micro satellite testing.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Sensorineural
Donor
Louis Reichardt, Ph.D., University of California, San Francisco.
Primary Reference
Muller U, Wang D, Denda S, Meneses JJ, Pedersen RA, Reichardt LF. Integrin alpha8beta1 is critically important for epithelial-mesenchymal interactions during kidney morphogenesis. Cell. 1997 Mar 7;88(5):603-13. (Medline PMID: 9054500)

Colony and Husbandry Information

Perinatal lethality resulting from failure to form kidney. Also deficit in hair cells in inner ear.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Heterozygous female x Wildtype male
  • Wildtype female x Heterozygous male
Generation
N1+F3 As of: 3/26/2004
Overall Breeding Performance
Good
Average litter size
7
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000193-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000193-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
000193-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.