Strain Detail Sheet

Strain Name    :

B6;129-Nfatc2tm1Rao/Mmnc

Stock Number :

000197-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Nfatc2tm1Rao
Name: targeted mutation 1, Anjana Rao
Chromosome: 2
Alteration at locus: Knockout
Gene Symbol: Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Chromosome: 2
Alteration at locus: Knockout

Genetic Alterations:
A neo cassette was inserted into an exon encoding amino acids near NH2 terminus of DNA binding domain.

Genotype Determination:

ES Cell Line: Not specified derived from B6;129S

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Complete loss of NFAT1 protein due to disrupted gene. Increased type II responses to inflammatory stimuli including eosinophilia and increased IgE levels and cytokine production.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Nfatc2tm1Rao/Nfatc2tm1Rao
        B6.Cg-Nfatc2<tm1Rao>
  • immune system phenotype
    • abnormal mast cell physiology (MGI Ref ID J:78089)
      • activated mast cells fail to produce IL-4 after stimulation
      • IL-13 and TNF production is also reduced
    • decreased interleukin-13 secretion (MGI Ref ID J:78089)
      • IL-13 production by activated mast cells is reduced
    • decreased interleukin-4 secretion (MGI Ref ID J:78089)
      • mast cells fail to produce IL-4 after stimulation
    • decreased tumor necrosis factor secretion (MGI Ref ID J:78089)
      • TNF production by activated mast cells is reduced

Strain of Origin: 129/Sv

Strain genetic background: C57BL/6

Strain Development: Founders on a mixed 129/SvJ and C57BL/6J background were crossed to C57BL/6 mice, subsequent heterozygotes were backcrossed to C57BL/6 for 4 generations giving a total of 5 backcrosses before sib mating was undertaken.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6;129-Nfatc2tm1Rao/Mmnc MMRRC:000197-UNC C57BL/6J 129S6 2548 34 0 0


Suggested Control Mice:

  • C57BL/6J

Research Applications

  • Immunology/Inflammation
  • Mast cells
  • Lymophcyte
  • Immune cell signaling and function
  • T-cell

Strain Origin

Donor: Dr. Anjana Rao, Center for Blood Research, Harvard University

Primary Reference:

  • Viola JP, Kiani A, Bozza PT, Rao A. Regulation of allergic inflammation and eosinophil recruitment in mice lacking the transcription factor NFAT1: role of interleukin-4 (IL-4) and IL-5. Blood. 1998 Apr 1;91(7):2223-30. (Medline PMID: 9516119)
  • Kiani A, Viola JP, Lichtman AH, Rao A. Down-regulation of IL-4 gene transcription and control of Th2 cell differentiation by a mechanism involving NFAT1. Immunity. 1997 Dec;7(6):849-60. (Medline PMID: 9430230

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Appearance

Coat color: Brown

Other:

Breeding

MMRRC Breeding System: Sib-mating

Breeding Scheme(s):

  • Homozygous female x Homozygous male

Generation: N4+

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: Unknown Unknown

Average litter size: 5

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000197-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000197-UNC-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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