Strain Name:
B6;129X1-Prkacatm1Gsm/Mmnc
Stock Number:
000199-UNC
Citation ID:
RRID:MMRRC_000199-UNC

Strain Information

Prkacatm1Gsm
Name: protein kinase, cAMP dependent, catalytic, alpha; targeted mutation 1, G Stanley McKnight
Synonyms: Calpha-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Prkaca
Name: protein kinase, cAMP dependent, catalytic, alpha
Synonyms: Cs, C alpha, PKA, Pkaca
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knockout
NCBI: 18747
HGNC: HGNC:9380
Homologene: 121574
Genetic Alterations
Exons 6-8 were replaced with a neomycin resistance cassette via homologous recombination. The knockout allele was confirmed via the absence of protein product in brains of homozygous mutant animals as determined by Western blot analysis.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    REK3 derived from 129X1/SvJ
    Phenotype
    Homozygotes experience early postnatal lethality with a few runted individuals (27 percent) surviving to early adulthood.
    Mammalian Phenotype Terms
    Allelic Composition: Prkacatm1Gsm/Prkacatm1Gsm (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

    Allelic Composition: Prkacatm1Gsm/Prkaca+ (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

    MeSH Terms
    • Animals
    • Brain/enzymology
    • Cyclic AMP-Dependent Protein Kinases/deficiency
    • Cyclic AMP-Dependent Protein Kinases/genetics
    • Cyclic AMP-Dependent Protein Kinases/physiology
    • Growth Disorders/enzymology
    • Infertility, Male/enzymology
    • Insulin-Like Growth Factor I/genetics
    • Kidney/metabolism
    • Liver/chemistry
    • Male
    • Mice
    • Mice, Knockout
    • Muscle, Skeletal/enzymology
    • Mutagenesis
    • Myocardium/enzymology
    • Protein Biosynthesis
    • RNA, Messenger/analysis
    • Sperm Motility/genetics
    • Spermatogenesis/genetics
    • Spermatozoa/enzymology
    • Spermatozoa/physiology
    Strain Development
    Chimeric mice were created using ES cell clones containing the targeted locus and then bred to produce heterozygotes lacking one functional C alpha gene. Homozygous survival rate increased on a 50:50 C57BL/6;129/SvJ background.
    Suggested Control Mice
    • Wildtype littermates
    • C57BL/6J
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Provides a model to study the absence of protein kinase A in most tissues
    Donor
    G. Stanley McKnight, Ph.D., University of Washington
    Primary Reference

    Skalhegg BS, Huang Y, Su T, Idzerda RL, McKnight GS, Burton KA. Mutation of the Calpha subunit of PKA leads to growth retardation and sperm dysfunction. Mol Endocrinol. 2002 Mar;16(3):630-9. (Medline PMID: 11875122)

    Colony and Husbandry Information

    75 percent of homozygotes die at early postnatal period. The few that survive are runted and can't be weaned from the mother until 3-4 months of age. They don't survive much past 6 months of age.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Various
    MMRRC Breeding System
    Backcross
    Breeding Scheme(s)
    • Heterozygous Female x C57BL/6 Male
    • C57BL/6 Female x Heterozygous Male
    Generation
    Unknown
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Reduced Reduced 75% of homozygotes die at early postnatal period. The few that survive are runted and can't be weaned from mother until 3-4 months of age. They don't survive much past 6 months of age.
    Homozygotes are fertile: Unknown Unknown
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Average litter size
    6-7
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    000199-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.