Strain Name    : B6.Cg-Sod2tm1Cje/Mmmh

Stock Number : 000202-MU

Gene Information

(provided by MGI)
Allele Symbol: Sod2tm1Cje
Name: targeted mutation 1, Charles J Epstein
Chromosome: 17
Alteration at locus: Knockout
Gene Symbol: Sod2
Name: superoxide dismutase 2, mitochondrial
Chromosome: 17
Alteration at locus: Knockout
Genetic Alterations:
The 3rd exon of Sod2 was replaced with Pol IIneo.

Genotype Determination:

ES Cell Line:

Phenotype

The heterozygous mutants have no overt phenotype. The homozygous mutants have an early lethal phenotype. About 60% of the homozygous mutants die around E15, the other live to term but usually die within 24 hours after thay are born.

Mammalian Phenotype Terms:
Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: B6.Cg-Sod2tm1Cje/Mmmh )

Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: D2.Cg-Sod2tm1Cje )

Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: involves: C57BL/6J )

Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: involves: C57BL/6J * CD-1 )

Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: involves: C57BL/6J * DBA/2J )

Allelic Composition: Sod2tm1Cje/Sod2+ (Genetic Background: B6.Cg-Sod2tm1Cje/Mmmh )

Allelic Composition: Sod2tm1Cje/Sod2+ (Genetic Background: D2.Cg-Sod2tm1Cje )

Allelic Composition: Sod2tm1Cje/Sod2+ (Genetic Background: involves: C57BL/6J * CD-1 )

Allelic Composition: Sod2tm1Cje/Sod2+ (Genetic Background: involves: C57BL/6J * DBA/2J )

Strain of Origin: CD1/Rb32/Rb2H

Strain genetic background: C57BL/6J

Suggested Control Mice:

  • Wildtype littermates

  • Apoptosis
  • Cardiovascular
  • Cell Biology
  • Diabetes/Obesity
  • Hematology
  • Metabolism
  • Models for Human Disease
  • Neurobiology

Donor: Ting-Ting Huang, Ph.D., Stanford University School of Medicine.

Primary Reference:

  • Li Y, Huang TT, Carlson EJ, Melov S, Ursell PC, Olson JL, Noble LJ, Yoshimura MP, Berger C, Chan PH, Wallace DC, Epstein CJ. Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase. Nat Genet. 1995 Dec;11(4):376-81. (Medline PMID: 7493016)
  • Huang TT, Carlson EJ, Kozy HM, Mantha S, Goodman SI, Ursell PC, Epstein CJ. Genetic modification of prenatal lethality and dilated cardiomyopathy in Mn superoxide dismutase mutant mice. Free Radic Biol Med. 2001 Nov 1;31(9):1101-10. (Medline PMID: 11677043)
  • Williams MD, Van Remmen H, Conrad CC, Huang TT, Epstein CJ, Richardson A. Increased oxidative damage is correlated to altered mitochondrial function in heterozygous manganese superoxide dismutase knockout mice. J Biol Chem. 1998 Oct 23;273(43):28510-5. PMID: 9774481 (Medline PMID: 9774481)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Black

Other:

MMRRC Breeding System: Intra strain mating

Breeding Scheme(s):

  • Heterozygous female x Wildtype male
  • Wildtype female x Heterozygous male

Generation: N21+

Overall Breeding Performance: Normal

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 16 weeks 16 weeks

Average litter size: 6 - 8

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
000202-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000202-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.