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Strain Name:
B6.Cg-Sod2tm1Cje/Mmmh
Stock Number:
000202-MU
Citation ID:
RRID:MMRRC_000202-MU

Strain Information

Sod2tm1Cje
Name: superoxide dismutase 2, mitochondrial; targeted mutation 1, Charles J Epstein
Synonyms: Sod2tm1Bay, Sod2mlucsf, Sod2m1ucsf, MnSOD, Sod2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knockout
Sod2
Name: superoxide dismutase 2, mitochondrial
Synonyms: MnSOD, manganese SOD, manganese superoxide dismutase, Sod-2
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Knockout
NCBI: 20656
VEGA: 17
Homologene: 530
Genetic Alterations
The 3rd exon of Sod2 was replaced with Pol II neo.
Genotype Determination
ES Cell Line
CB1-4 derived from C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1
Phenotype
The heterozygous mutants have no overt phenotype. The homozygous mutants have an early lethal phenotype. About 60% of the homozygous mutants die around E15, the other 40% live to term but usually die within 24 hours after birth.
Mammalian Phenotype Terms
Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: B6.Cg-Sod2tm1Cje/Mmmh )

Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: D2.Cg-Sod2tm1Cje )

Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: involves: C57BL/6J )

Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: involves: C57BL/6J * CD-1 )

Allelic Composition: Sod2tm1Cje/Sod2tm1Cje (Genetic Background: involves: C57BL/6J * DBA/2J )

Allelic Composition: Sod2tm1Cje/Sod2+ (Genetic Background: B6.Cg-Sod2tm1Cje/Mmmh )

Allelic Composition: Sod2tm1Cje/Sod2+ (Genetic Background: D2.Cg-Sod2tm1Cje )

Allelic Composition: Sod2tm1Cje/Sod2+ (Genetic Background: involves: C57BL/6J * CD-1 )

Allelic Composition: Sod2tm1Cje/Sod2+ (Genetic Background: involves: C57BL/6J * DBA/2J )

MeSH Terms
  • Acidosis/enzymology
  • Animals
  • Animals, Newborn
  • Base Sequence
  • Cardiomyopathy, Dilated/enzymology
  • Cardiomyopathy, Dilated/genetics
  • Electron Transport Complex IV/analysis
  • Gene Targeting
  • Genes, Lethal
  • Homozygote
  • Lipid Peroxidation
  • Lipids/analysis
  • Liver/chemistry
  • Mice
  • Mice, Mutant Strains
  • Mitochondria, Heart/enzymology
  • Mitochondria, Heart/ultrastructure
  • Mitochondria, Muscle/enzymology
  • Mitochondria, Muscle/ultrastructure
  • Molecular Sequence Data
  • Muscle, Skeletal/chemistry
  • Sequence Deletion
  • Succinate Dehydrogenase/analysis
  • Superoxide Dismutase/genetics
  • Superoxide Dismutase/metabolism
  • Acidosis/genetics
  • Acidosis/metabolism
  • Aconitate Hydratase/genetics
  • Aconitate Hydratase/metabolism
  • Catalase/genetics
  • Catalase/metabolism
  • Fetal Death/genetics
  • Fetal Death/metabolism
  • Genotype
  • Glutathione Peroxidase/genetics
  • Glutathione Peroxidase/metabolism
  • Kidney/metabolism
  • Lipid Metabolism
  • Liver/metabolism
  • Mice, Knockout
  • Mitochondria/metabolism
  • Myocardium/cytology
  • Myocardium/metabolism
  • Phenotype
  • Superoxide Dismutase/deficiency
  • Up-Regulation
  • Aconitate Hydratase/analysis
  • Heterozygote
  • Mitochondria, Liver/metabolism
  • Oxidative Stress/physiology
  • Oxygen Consumption
  • Permeability
  • Reactive Oxygen Species/metabolism
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
  • Apoptosis
  • Cardiovascular
  • Cell Biology
  • Diabetes/Obesity
  • Hematology
  • Metabolism
  • Models for Human Disease
  • Neurobiology
Donor
Ting-Ting Huang, Ph.D., Stanford University School of Medicine.
Primary Reference
  • Li Y, Huang TT, Carlson EJ, Melov S, Ursell PC, Olson JL, Noble LJ, Yoshimura MP, Berger C, Chan PH, Wallace DC, Epstein CJ. Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase. Nat Genet. 1995 Dec;11(4):376-81. (Medline PMID: 7493016)
  • Huang TT, Carlson EJ, Kozy HM, Mantha S, Goodman SI, Ursell PC, Epstein CJ. Genetic modification of prenatal lethality and dilated cardiomyopathy in Mn superoxide dismutase mutant mice. Free Radic Biol Med. 2001 Nov 1;31(9):1101-10. (Medline PMID: 11677043)
  • Williams MD, Van Remmen H, Conrad CC, Huang TT, Epstein CJ, Richardson A. Increased oxidative damage is correlated to altered mitochondrial function in heterozygous manganese superoxide dismutase knockout mice. J Biol Chem. 1998 Oct 23;273(43):28510-5. PMID: 9774481 (Medline PMID: 9774481)
Strain Development


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Intra strain mating
Breeding Scheme(s)
  • Heterozygous female x Wildtype male
  • Wildtype female x Heterozygous male
Generation
N21+
Overall Breeding Performance
Normal
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 16 weeks 16 weeks
Average litter size
6 - 8
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000202-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
000202-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.