Strain Name    : B6.129X1-Wrntm1Lgu/Mmnc

Stock Number : 000204-UNC

Gene Information

(provided by MGI)
Allele Symbol: Wrntm1Lgu
Name: targeted mutation 1, Leonard Guarente
Chromosome: 8
Alteration at locus: Knockout
Gene Symbol: Wrn
Name: Werner syndrome homolog (human)
Chromosome: 8
Alteration at locus: Knockout
Genetic Alterations:
The 3'-most exon of the helicase domain-encoding region was replaced with a beta-geo casette (beta-gal/neo fusion).

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line:

    Phenotype

    Homozyotes WRN mutants are healthy, fertile and have a normal lifespan. Homozyogous WRN mutation accelerates mortality in p53-/- background.

    Mammalian Phenotype Terms:
    Allelic Composition: Wrntm1Lgu/Wrntm1Lgu (Genetic Background: involves: BALB/c )

    Strain of Origin: 129X1/SvJ

    Strain genetic background: C57BL/6J

    Strain Development:

    Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC StrainReference StrainsGenomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    B6.129X1-Wrntm1Lgu/Mmnc MMRRC:000204-UNC C57BL/6J 129X1 2563 0 18 0


    Suggested Control Mice: Wild-type littermates or C57BL/6

    • Apoptosis
    • Cancer
    • Models for Human Disease
    • Aging

    Donor: Brad Johnson, M.D., Ph.D., University of Pennsylvania.

    Primary Reference:

    Lombard DB, Beard C, Johnson B, Marciniak RA, Dausman J, Bronson R, Buhlmann JE, Lipman R, Curry R, Sharpe A, Jaenisch R, Guarente L. Mutations in the WRN gene in mice accelerate mortality in a p53-null background. Mol Cell Biol. 2000 May;20(9):3286-91. (Medline PMID: 10757812)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Coat color: Black

    Other:

    MMRRC Breeding System: Backcross

    Breeding Scheme(s):

    • C57BL/6J female x Knockout male

    Generation: N12 As of: 11/2/04

    Overall Breeding Performance: Good

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: Unknown Unknown
    Homozygotes are fertile: Unknown Unknown
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown

    Average litter size: 7

    Recommended wean age: 3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    000204-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    000204-UNC-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2
    000204-UNC-EMBRYOCryo-preserved embryos
    $1,038.00
    Non-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.