Strain Name:
C.129S2(B6)-Ighmtm1Cgn/Mmmh
Stock Number:
000205-MU
Citation ID:
RRID:MMRRC_000205-MU

Strain Information

Ighmtm1Cgn
Name: immunoglobulin heavy constant mu; targeted mutation 1, University of Cologne
Synonyms: mum-, Cmu -, IgH-, Igh-6tm1Cgn, Igmunull, BKO, Igh-6-, muIgKO, muMt-, BCKO, Igh-6null, Ighm-, Ig- muMT, B cell-, IgHmuMT, Igh-6tm1CgnmuMT0, mu-MT-, muMT, Ig-, BCR-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Ighm
Name: immunoglobulin heavy constant mu
Synonyms: muH, Igh6, Ig mu, IgM, Igh-M
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knockout
NCBI: 16019
HGNC: HGNC:5541
Genetic Alterations
A neo cassette was inserted close to the 5' boundary of the first exon of Igh-6.
Genotype Determination
ES Cell Line
D3 derived from 129S2/SvPas
Phenotype
The mice lack expression of mature B-CELL. They produce no membrane-bound IgM.
Mammalian Phenotype Terms
Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: B6.129S2-Ighmtm1Cgn )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: B6.129S2-Ighmtm1Cgn/J )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: involves: 129S2/SvPas )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: NOD.129S2-Ighmtm1Cgn )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: NOD.129S2-Ighmtm1Cgn/DvsJ )

Allelic Composition: Ighmtm1Cgn/Ighmtm1Cgn (Genetic Background: NODCaj.129S2-Ighmtm1Cgn )

Allelic Composition: Ighmtm1Cgn/Ighm+ (Genetic Background: involves: 129S2/SvPas * C57BL/6 )

Allelic Composition: Ighmtm1Cgn/Ighm+ (Genetic Background: NOD.129S2-Ighmtm1Cgn )

MeSH Terms
  • Animals
  • Animals, Newborn/genetics
  • Animals, Newborn/immunology
  • Antibodies, Anti-Idiotypic/administration & dosage
  • Antibodies, Anti-Idiotypic/genetics
  • Antigen Presentation/genetics
  • Autoimmune Diseases/genetics
  • Autoimmune Diseases/immunology
  • Autoimmune Diseases/pathology
  • B-Lymphocytes/immunology
  • B-Lymphocytes/pathology
  • Immunoglobulin M/biosynthesis
  • Immunoglobulin M/immunology
  • Immunoglobulin mu-Chains/genetics
  • Immunoglobulin mu-Chains/immunology
  • Injections, Intraperitoneal
  • Lymphocyte Depletion
  • Lymphopenia/genetics
  • Lymphopenia/immunology
  • Lymphopenia/pathology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myocarditis/genetics
  • Myocarditis/immunology
  • Myocarditis/pathology
  • Myosins/administration & dosage
  • Myosins/immunology
Strain Development
The original mutation is on a B6;129S2 background. A congenic strain was made by backcrossing onto a BALB/cJ background and then maintained by intercrossing after 9 generations of backcrossing. The mu chain knockout gene is homozygous in this congenic strain.
Suggested Control Mice
  • BALB/c
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Autoimmunity
  • Cardiovascular
  • Immunology and Inflammation
  • Immunodeficiency
  • Research Tools
  • Virology
  • Useful for determining the role that B cells play in autoimmune disease and infectious disease studies for which BALB/c mice are the susceptible strain.
Donor
Dr. Beth Bauer, University of Missouri
Primary Reference
Malkiel S, Factor S, Diamond B. Autoimmune myocarditis does not require B cells for antigen presentation. J Immunol. 1999 Nov 15;163(10):5265-8. (Medline PMID: 10553048)

Colony and Husbandry Information

Separate pregnant females to individual cages prior to birth for maximum pup survival. Mice do not produce antibodies, so their immune system is compromised. Caging, food and water should be sterilized, and cage changes should be done within a laminar flow station.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Albino
MMRRC Breeding System
Inbred
Breeding Scheme(s)
Homozygous female x Homozygous male
Generation
N9 prior to sib mating. F16 As of: 3/2003
Overall Breeding Performance
Good
Average litter size
5
Recommended wean age
4 weeks (few pups weaned at 21 days survive)

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000205-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000205-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
000205-MU-RESUS Litter recovered from cryo-archive $2,028.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.