Strain Name:
B6;FVB-Tg(Dspp-cre)1Kul/Mmucd
Stock Number:
000206-UCD
Citation ID:
RRID:MMRRC_000206-UCD
Other Names:
DSPP-Cre, B6;FVB-Tg(Dspp-cre)1Kul/Mmcd

Strain Information

cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Tg(Dspp-cre)1Kul
Name: transgene insertion 1, Ashok B Kulkarni
Synonyms: Dspp-Cre
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genetic Alterations
Random Integration
Genotype Determination
Phenotype
The expression of Cre recombinase protein was mainly detected in the nucleus of the odontoblasts. The efficiency of Cre activity was analyzed by crossing the Dspp-Cre mice with ROSA26 reporter (R26R) mice. The offspring with both genotypes have shown specific deletion of intervening sequences flanked by loxP sites upstream of the reporter gene, thereby facilitating the expression of the  -galactosidase ( -gal) gene in the teeth. The activity of  -gal was initially observed in the odontoblasts of 1-day-old mice and increased with tooth development. Almost all of the odontoblasts have shown lacZ activity by 3 weeks of age.
MeSH Terms
  • Animals
  • Extracellular Matrix Proteins
  • Gene Deletion
  • Genes, Reporter
  • Genetic Techniques
  • Genotype
  • Immunohistochemistry
  • Integrases/biosynthesis
  • Integrases/genetics
  • Mice
  • Mice, Transgenic
  • Models, Genetic
  • Odontoblasts/metabolism
  • Phosphoproteins
  • Promoter Regions, Genetic
  • Protein Precursors/genetics
  • Sialoglycoproteins
  • Time Factors
  • Tooth/embryology
  • Viral Proteins/biosynthesis
  • Viral Proteins/genetics
  • beta-Galactosidase/metabolism
Strain Development
The transgenic construct was generated by cloning the Sal I-Nru I fragment spanning the dspp upstream sequences and the 1.6 kb Xba I blunted-Sal I fragments into the Sal I digested and dephosphorylated pBS II SK vector . Founder mice were generated by microinjecting 7.6 kb Sal I fragment into the pronucleus of fertilized FVB/N mouse zygotes. The presence of the transgene was identified by Southern blot analysis of tail DNA using 32Plabeled SV40 poly A sequences as a probe. Since transfer to MMRRC this strain has been further backcrossed to C57BL/6J to produce the congenic strain, B6.FVB-Tg(Dspp-cre)1Kul/Mmcd (see MMRRC #15967), that is 98% C57BL/6J by micro satellite testing.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Research Tools
Donor
Taduru Sreenath, Ph.D., National Institutes of Health/National Institute of Dental and Craniofacial Research
Ashok Kulkarni, Ph.D., National Institutes of Health/National Institute of Dental and Craniofacial Research
Primary Reference
Sreenath TL, Cho A, Thyagarajan T, Kulkarni AB. Odontoblast-specific expression of cre recombinase successfully deletes gene segments flanked by loxP sites in mouse teeth. Genesis. 2003 Feb;35(2):94-9. (Medline PMID: 12533791)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti
MMRRC Breeding System
Inbreeding; backcross
Breeding Scheme(s)
  • Hemizygous female x Wildtype male
  • Wildtype female x Hemizygous male
  • C57BL/6J female x Hemizygous male
  • Hemizygous female x C57BL/6J male
Generation
N6F3 As of: 5/1/2004
Overall Breeding Performance
Good
Average litter size
10 - 12
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000206-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000206-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
000206-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.