Strain Name    : B6.129S7-Chrnb2tm1Mdb/Mmnc

Stock Number : 000211-UNC

Gene Information

(provided by MGI)
Allele Symbol: Chrnb2tm1Mdb
Name: targeted mutation 1, Mariella De Biasi
Chromosome: 3
Alteration at locus: Knockout
Gene Symbol: Chrnb2
Name: cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)
Chromosome: 3
Alteration at locus: Knockout
Genetic Alterations:
A neomycin cassette replaces exons 1-5.

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line:

    Phenotype

    Homozygous mice: beta2 nAChR null mutant mice do not become addicted to nicotine, are less sensitive to nicotine's antinociceptive effects and show neuronal loss during ageing.

    Heterozygous mice: There is about a 50% loss of high affinity nicotine binding sites in the CNS.

    Mammalian Phenotype Terms:
    Allelic Composition: Chrnb2tm1Mdb/Chrnb2tm1Mdb (Genetic Background: involves: 129S7/SvEvBrd )

    Allelic Composition: Chrnb2tm1Mdb/Chrnb2tm1Mdb (Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J )

    Strain of Origin: 129/SvEv

    Strain genetic background: C57BL/6J

    Strain Development: The mouse gene for the nAChR b2 subunit was isolated by screening a mouse 129/SvEv genomic library with a rat cDNA probe (Wada et al., 1989). The isolated genomic clone contained the region from exons 1 to 6, and the detailed restriction map and intron and exon boundaries were obtained. A construct with the 8 kb region from exons 1 to 5, which contains three out of the four transmembrane domains, replaced with a neomycin resistance cassette (Neo) was electroporated into the AB2.1 embryonic stem (ES) cells and transmitted into the germline as described previously (Bradley, 1987; Xu et al., 1999). Chimeric mice were bred with C57BL/6J mice.

    • Models for Human Disease
    • Neurobiology

    Donor: Art Beaudet, M.D., Baylor College of Medicine

    Primary Reference:

    • Xu W, Orr-Urtreger A, Nigro F, Gelber S, Sutcliffe CB, Armstrong D, Patrick JW, Role LW, Beaudet AL, De Biasi M. Multiorgan autonomic dysfunction in mice lacking the beta2 and the beta4 subunits of neuronal nicotinic acetylcholine receptors. J Neurosci. 1999 Nov 1;19(21):9298-305. (Medline PMID: 10531434)
    • Marubio LM, del Mar Arroyo-Jimenez M, Cordero-Erausquin M, Lena C, Le Novere N, de Kerchove d'Exaerde A, Huchet M, Damaj MI, Changeux JP. Reduced antinociception in mice lacking neuronal nicotinic receptor subunits. Nature. 1999 Apr 29;398(6730):805-10. (Medline PMID: 10235262)
    • Picciotto MR, Zoli M, Rimondini R, Lena C, Marubio LM, Pich EM, Fuxe K, Changeux JP. Acetylcholine receptors containing the beta2 subunit are involved in the reinforcing properties of nicotine. Nature. 1998 Jan 8;391(6663):173-7. (Medline PMID: 9428762)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Coat color: Black

    Other:

    MMRRC Breeding System: Backcross

    Breeding Scheme(s):

    • Backcross to C57BL/6J

    Generation: N11

    Overall Breeding Performance: Normal

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
     
    Age Reproductive Decline: 16 weeks 16 weeks

    Average litter size: 6 - 8

    Recommended wean age: 3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    000211-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    000211-UNC-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.