Allele Symbol: Chrnb2tm1Mdb
Name: targeted mutation 1, Mariella De Biasi
Alteration at locus: Knockout
Gene Symbol: Chrnb2
Name: cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)
Alteration at locus: Knockout
ES Cell Line:
Homozygous mice: beta2 nAChR null mutant mice do not become addicted to nicotine,
are less sensitive to nicotine's antinociceptive effects and show
neuronal loss during ageing.
Heterozygous mice: There is about a 50% loss of high affinity nicotine binding sites in the CNS.
Strain of Origin: 129/SvEv
Strain genetic background: C57BL/6J
Strain Development: The mouse gene for the nAChR b2 subunit was isolated by screening a mouse 129/SvEv genomic library with a rat cDNA probe (Wada et al., 1989). The isolated genomic clone contained the region from exons 1 to 6, and the detailed restriction map and intron and exon boundaries were obtained. A construct with the 8 kb region from exons 1 to 5, which contains three out of the four transmembrane domains, replaced with a neomycin resistance cassette (Neo) was electroporated into the AB2.1 embryonic stem (ES) cells and transmitted into the germline as described previously (Bradley, 1987; Xu et al., 1999). Chimeric mice were bred with C57BL/6J mice.
Donor: Art Beaudet, M.D., Baylor College of Medicine
Coat color: Black
MMRRC Breeding System: Backcross
Overall Breeding Performance: Normal
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||16 weeks||16 weeks|
Average litter size: 6 - 8
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.