Strain Name:
STOCK Mafbm1Gsb/Mmucd
Stock Number:
000022-UCD
Citation ID:
RRID:MMRRC_000022-UCD
Other Names:
STOCK Mafbm1Gsb/Mmcd, STOCK Mafbenu1 (Allele designation correction), Kr/+

Strain Information

Mafbm1Gsb
Name: MAF bZIP transcription factor B; mutation 1, Gregory S Barsh
Synonyms: MafBENU, krENU, kreislerenu
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
Mafb
Name: MAF bZIP transcription factor B
Synonyms: Krml, Krml1, Kreisler
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16658
HGNC: HGNC:6408
Homologene: 31315
Genetic Alterations
A G to A transition causes an Asn to Ser substitution in DNA binding domain, and abolishes ability to bind target DNA sequence.
Genotype Determination
Phenotype
This is a null allele of the target gene, which is an inversion that causes tissue-specific loss of hindbrain expression, but preserves expression in other tissues. The homozygous kreisler mouse and trans heterozygotes for the ENU allele show an identical inner ear phenotype due to rhombomere segmentation abnormalities, but the homozygous ENU allele has additional abnormalities due to a requirement for the target gene in tissues other than the hindbrain.
  • Failure of rhombomere segmentation
  • Neonatal death
  • Kidney defect
Mammalian Phenotype Terms
Allelic Composition: Mafbm1Gsb/Mafbm1Gsb (Genetic Background: B6.Cg-Mafbm1Gsb )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Avian Proteins
  • Base Sequence
  • Chromosome Inversion
  • Cloning, Molecular
  • DNA-Binding Proteins/genetics
  • Ear/embryology
  • Female
  • In Situ Hybridization
  • Leucine Zippers
  • MafB Transcription Factor
  • Male
  • Mice
  • Mice, Mutant Strains/embryology
  • Models, Biological
  • Molecular Sequence Data
  • Mutation
  • Oncogene Proteins
  • Point Mutation
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins c-maf
  • RNA, Messenger/analysis
  • Restriction Mapping
  • Rhombencephalon/abnormalities
  • Sequence Homology, Amino Acid
  • Transcription Factors/genetics
  • Transcription, Genetic
Strain Development
The ENU mutant allele was induced on (BALB/C x SJL/J)F1 then mated to (C57BL/6 x C3H)F1 and subsequently backcrossed to C57BL/6J. Currently being maintained by brother-sister mating of heterozyote X wildtype.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Brain Dysmorphology
Donor
Dr. Greg Barsh, MD PhD., Stanford University
Primary Reference

Cordes SP, Barsh GS. The mouse segmentation gene kr encodes a novel basic domain-leucine zipper transcription factor. Cell. 1994 Dec 16;79(6):1025-34. (Medline PMID: 8001130)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black/brown (may vary)
MMRRC Breeding System
Sibling mating or Backcross
Breeding Scheme(s)
  • Heterozygous female x Wildtype male
  • Wildtype female x Heterozygous male
Generation
N2F1
Overall Breeding Performance
Good
Average litter size
4
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000022-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000022-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
000022-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.