Strain Name:
STOCK Plntm1Egk/Mmmh
Stock Number:
000027-MU
Citation ID:
RRID:MMRRC_000027-MU

Strain Information

Plntm1Egk
Name: phospholamban; targeted mutation 1, Evangelia G Kranias
Synonyms: PLN-, PLB-, PLB-KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Pln
Name: phospholamban
Synonyms: PLB
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knockout
NCBI: 18821
VEGA: 10
HGNC: HGNC:9080
Homologene: 2002
Genetic Alterations
Neo cassette was inserted into exon 2.
Genotype Determination
ES Cell Line
D3 derived from 129S2/SvPas
Phenotype
Enhanced myocardial calcium handling and contractile parameters without changes in heart rate.
Mammalian Phenotype Terms
Allelic Composition: Plntm1Egk/Plntm1Egk (Genetic Background: involves: 129S2/SvPas )

Allelic Composition: Plntm1Egk/Plntm1Egk (Genetic Background: involves: 129S2/SvPas * CF-1 * FVB/N )

Allelic Composition: Plntm1Egk/Plntm1Egk (Genetic Background: involves: 129S2/SvPas * CF1 )

MeSH Terms
  • Animals
  • Blastocyst/physiology
  • Blotting, Western
  • Calcium/metabolism
  • Calcium-Binding Proteins/biosynthesis
  • Calcium-Binding Proteins/genetics
  • Calcium-Transporting ATPases/metabolism
  • Cardiac Output/drug effects
  • Cloning, Molecular
  • Embryo, Mammalian
  • Female
  • Gene Deletion
  • Genomic Library
  • Heart/drug effects
  • Heart/physiology
  • In Vitro Techniques
  • Isoproterenol/pharmacology
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Myocardial Contraction
  • Myocardium/metabolism
  • Pseudopregnancy
  • Restriction Mapping
  • Sarcoplasmic Reticulum/metabolism
  • Stem Cells/physiology
Strain Development
Phospholamban-targeted ES cells were injected into C57BL/6 blastocysts and transferred to F1(C3H HeN x C57BL/6J) pseudopregnant females. Coat-color offspring were mated to albino nonagouti CF-1 mice. Resulting heterozygous mice carrying the targeted phospholamban allele were mated to produce homozygous mutant mice completely deficient in phospholamban.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Elucidate cellular mechanisms involved in the regulation of calcium cycling and contractility in the cardiac, smooth and slow-twitch skeletal muscles in health and disease.
  • Screen potential therapeutic targets in the treatment of heart failure or other muscle diseases with disturbed calcium homeostasis.
Donor
Evangelia Kranias, Ph.D., University of Cincinnati, College of Medicine.
Primary Reference
Luo W, Grupp IL, Harrer J, Ponniah S, Grupp G, Duffy JJ, Doetschman T, Kranias EG. Targeted ablation of the phospholamban gene is associated with markedly enhanced myocardial contractility and loss of beta-agonist stimulation. Circ Res. 1994 Sep;75(3):401-9. (Medline PMID: 8062415)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Homozygous Female x Homozygous Male
Generation
Unspecified
Overall Breeding Performance
Good. However, older breeding males may become aggressive.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6-7 months 6-7 months
Bred to Homozygosity
Yes
Average litter size
8
Recommended wean age
3 weeks
Average Pups Weaned
7

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000027-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
000027-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.