Strain Name:
B6;129-Kifc3tm1Gsn/Mmmh
Stock Number:
000028-MU
Citation ID:
RRID:MMRRC_000028-MU

Strain Information

Kifc3tm1Gsn
Name: kinesin family member C3; targeted mutation 1, Lawrence SB Goldstein
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Kifc3
Name: kinesin family member C3
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knockout
NCBI: 16582
HGNC: HGNC:6326
Homologene: 124421
Genetic Alterations
6.5 kb DNA fragment (aa 197-507) was replaced with a SA-IRES-beta-geo cassette.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Analysis to date shows null mutants behaving as wildtype littermate controls. C3 kinesin is an ubiquitously expressed molecular motor. Additional analysis of its functions in yet uncharacterized systems may be insightful into its role in intracellular transport.
Mammalian Phenotype Terms
Allelic Composition: Kifc3tm1Gsn/Kifc3tm1Gsn (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary/genetics
  • Female
  • Gene Expression
  • Growth/genetics
  • Growth/physiology
  • Kidney/anatomy & histology
  • Kidney/metabolism
  • Kinesin/chemistry
  • Kinesin/genetics
  • Kinesin/physiology
  • Male
  • Mice
  • Mice, Knockout
  • Molecular Motor Proteins/chemistry
  • Molecular Motor Proteins/genetics
  • Molecular Motor Proteins/physiology
  • Molecular Sequence Data
  • Phenotype
  • Pregnancy
  • Reproduction/genetics
  • Reproduction/physiology
  • Retina/metabolism
Strain Development
R1 ES cells injected into C57BL/6 blastocysts and resulting chimerics crossed to C57BL/6; the heterozygous agouti offspring were inbred to develop homozygous animals.
Suggested Control Mice
  • C57BL/6
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Intracellular transport mechanisms
Donor
Dr. Larry Goldstein, University of California, San Diego
Primary Reference

Yang Z, Xia Ch, Roberts EA, Bush K, Nigam SK, Goldstein LS. Molecular cloning and functional analysis of mouse C-terminal kinesin motor KifC3. Mol Cell Biol. 2001 Feb;21(3):765-70. (Medline PMID: 11154264)

Colony and Husbandry Information

Remove male prior to birth of pups; female will neglect or cannibalize litter if male is in the cage at time of birth.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Various (black, agouti, albino)
MMRRC Breeding System
Sib mating
Breeding Scheme(s)
  • Homozygous Female x Homozygous Male
  • Heterozygous Female x Heterozygous Male
Generation
Unknown
Overall Breeding Performance
Excellent
Average litter size
8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000028-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000028-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
000028-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.