Name: targeted mutation 2, Henry A Lester
Alteration at locus: Knock-In
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Alteration at locus: Knock-In
ES Cell Line: Not specified derived from B6;129S1
Homozygous: Viable and fertile homozygous behavioural hypersensitivity to Nicotinic agonists
Heterozygous: Viable, fertile, anxious and hypersensitive to Nicotine induced seizers.
Strain of Origin: 129/ SvJ
Strain genetic background: C57BL/6
Strain Development: Donor took a complementary approach to the KO strategy by replacing an endogenous exon with one containing a single point mutation, Leu9' Y Ala9' (Leu9'Ala), within the putative pore-forming M2 domain of the a4 subunit, rendering a4* receptors hypersensitive to nicotine.
Suggested Control Mice: Wild-type littermates
Donor: Henry A. Lester, Ph.D., CALTECH
MMRRC Breeding System:
Overall Breeding Performance:
Average litter size:
Recommended wean age:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.