Strain Detail Sheet

Strain Name    :

B6.129S1-Chrna4tm2Lst/Mmucd

Stock Number :

029171-UCD

Other Names   :

C57BL/6a4L9'A-D/DC6H4/bx, B6.129S1-Chrna4tm2Lst/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Target: Chrna4tm2Lst
Name: targeted mutation 2, Henry A Lester
Alteration at locus: Knock-In
Target: Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Chromosome: 2
Alteration at locus: Knock-In

Genetic Alterations:
129/SvJ α4 genomic clone containing exon 5 with the L9

Genotype Determination:

ES Cell Line: Not specified derived from B6;129S1

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous: Viable and fertile homozygous behavioural hypersensitivity to Nicotinic agonists

Heterozygous: Viable, fertile, anxious and hypersensitive to Nicotine induced seizers.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Chrna4tm2Lst/Chrna4tm2Lst
        involves: 129S1/Sv * C57BL/6
  • behavior/neurological phenotype
    • enhanced behavioral response to nicotine (MGI Ref ID J:93873)
      • homozygotes require a 50-fold lower concentration of nicotine to induce conditioned place preference, hyperactivity, hypothermia, and to develop a tolerance to induced-hypothermia
      • a similar increase in sensitivity to nicotine is seen in dopaminergic neurons and in ventral midbrain neurons
    • enhanced conditioned place preference behavior (MGI Ref ID J:93873)

Strain of Origin: 129/ SvJ

Strain genetic background: C57BL/6

Strain Development: Donor took a complementary approach to the KO strategy by replacing an endogenous exon with one containing a single point mutation, Leu9' Y Ala9' (Leu9'Ala), within the putative pore-forming M2 domain of the a4 subunit, rendering a4* receptors hypersensitive to nicotine.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools

Strain Origin

Donor: Henry A. Lester, Ph.D., CALTECH

Primary Reference:

  • Tapper AR, McKinney SL, Nashmi R, Schwarz J, Deshpande P, Labarca C, Whiteaker P, Marks MJ, Collins AC, Lester HA. Nicotine activation of alpha4* receptors: sufficient for reward, tolerance, and sensitization. Science. 2004 Nov 5;306(5698):1029-32. (Medline PMID: 15528443)
  • Fonck C, Cohen BN, Nashmi R, Whiteaker P, Wagenaar DA, Rodrigues-Pinguet N, Deshpande P, McKinney S, Kwoh S, Munoz J, Labarca C, Collins AC, Marks MJ, Lester HA. Novel seizure phenotype and sleep disruptions in knock-in mice with hypersensitive alpha 4* nicotinic receptors. J Neurosci. 2005 Dec 7;25(49):11396-411. (Medline PMID: 16339034)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color:

Other:

Breeding

MMRRC Breeding System:

Breeding Scheme(s):

    Generation:

    Overall Breeding Performance:

    Reproductive Statistics

    Viability and Fertility: Unknown

    Average litter size:

    Recommended wean age:

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    029171-UCD-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    029171-UCD-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2
    029171-UCD-EMBRYOCryo-preserved embryos
    $1,038.00
    Non-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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    Generated: 04/24/2014