Strain Name:
B6;129X1-Slc39a2tm1Gka/Mmmh
Stock Number:
029176-MU
Citation ID:
RRID:MMRRC_029176-MU
Other Names:
Zip2

Strain Information

Slc39a2tm1Gka
Name: solute carrier family 39 (zinc transporter), member 2; targeted mutation 1, Glen K Andrews
Synonyms: Zip2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
Slc39a2
Name: solute carrier family 39 (zinc transporter), member 2
Synonyms: zip2, F730005G13Rik
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Knockout
NCBI: 214922
Homologene: 40957
Genetic Alterations
Using homologous recombination, exon 2 of the Zip2 gene was knocked out. Inserted was an EGFP reporter, followed by a floxed Neomycin cassette. The floxed Neo was later removed by crossing with mice expressing CRE recombinase.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Zinc deficient diet during pregnancy greatly increases the risk of abnormal development and deformities. Iron and calcium homeostasis is greatly affected in tissue-specific ways.

Heterozygous: Not characterized
Mammalian Phenotype Terms
Allelic Composition: Slc39a2tm1Gka/Slc39a2tm1Gka (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N )

MeSH Terms
  • Animals
  • Calcium/metabolism
  • Cation Transport Proteins/genetics
  • Cation Transport Proteins/physiology
  • Dendritic Cells/chemistry
  • Dendritic Cells/metabolism
  • Female
  • Gene Expression
  • Gene Expression Regulation, Developmental
  • Gene Targeting
  • Green Fluorescent Proteins/analysis
  • Green Fluorescent Proteins/genetics
  • Hepatocytes/chemistry
  • Hepatocytes/metabolism
  • Homeostasis
  • Iron/metabolism
  • Keratinocytes/chemistry
  • Keratinocytes/metabolism
  • Mice
  • Mice, Knockout
  • Pregnancy
  • Trophoblasts/chemistry
  • Trophoblasts/metabolism
  • Zinc/metabolism
Strain Development
Chimeric founders were outbred to C57BL/6 mice. Heterozygous offspring were inbred to generate homozygous knockouts. Homozygous knockouts were outbred to CRE-recombinase expressing mice. Heterozygous mice lacking the neomycin cassette were inbred to create homozygous knockout mice.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Developmental Biology
  • Immunology and Inflammation
  • Internal/Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Research Tools
Donor
Glen K. Andrews, Ph.D., Kansas University Medical Center
Primary Reference
Peters JL, Dufner-Beattie J, Xu W, Geiser J, Lahner B, Salt DE, Andrews GK. Targeting of the mouse Slc39a2 (Zip2) gene reveals highly cell-specific patterns of expression, and unique functions in zinc, iron, and calcium homeostasis. Genesis. 2007 Jun;45(6):339-52. (Medline PMID: 17506078)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
029176-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
029176-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.