Strain Detail Sheet

Strain Name    :

B6.Cg-Mir155tm1.1Brd Tyrc-Brd/Mmmh

Stock Number :

029594-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mir155tm1.1Brd
Name: targeted mutation 1.1, Allan Bradley
Alteration at locus: Knockout
Allele Symbol: Tyrc-Brd
Name: albino, Allan Bradley
Alteration at locus: Knockout
Gene Symbol: Mir155
Name: microRNA 155
Chromosome: 16
Alteration at locus: Knockout

Genetic Alterations:
A small deletion was created to remove exon 2 of the Bic/miR155 locus. This is a null allele for the locus.

Genotype Determination:

ES Cell Line: AB2.2 derived from 129S7/SvEvBrd-Hprt

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous: Immuno-compromised mice. Defective responses by B/T cells and dendritic cells.

Heterozygous mice have no phenotype.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Mir155tm1.1Brd/Mir155tm1.1Brd
        B6.Cg-Mir155<tm1.1Brd> Tyr<c-Brd>
  • immune system phenotype
    • immune system phenotype (MGI Ref ID J:121095)
      • no gross defects in myeloid or lymphoid development are observed in mutants
      • abnormal CD4-positive T cell morphology (MGI Ref ID J:121095)
        • number of Il-4 single producing cells is doubled in culture with nonpolarizing conditions (ThN)
        • abnormal T-helper 1 cell morphology (MGI Ref ID J:121095)
          • mutant Th1 cells produce elevated levels of CCL-5
        • increased T-helper 2 cell number (MGI Ref ID J:121095)
          • commitment to Th2 pathway is increased; number of Il-4 producing cells in is increased
      • abnormal CD4-positive T cell physiology (MGI Ref ID J:121095)
        • uncommitted CD4+ T cells show reduced Ifng production after stimulation with CD3 and CD28 antibodies
        • Ifng-producing cells are reduced 5-fold under sulture conditions nonpolarizing for T helper responses; under Th2 polarizing conditions Il-4, -5 and -10 production is elevated
      • abnormal dendritic cell physiology (MGI Ref ID J:121095)
        • bone marrow-derived dendritic cells from mutants fail to efficiently activate T cells, but show normal levels of MHC-II and CD86 when stimulated with LPS in culture
      • decreased IgG1 level (MGI Ref ID J:121095)
        • production of IgG1 by LPS and Il-4 stimulation of B cells is significantly reduced relative to controls
      • increased susceptibility to bacterial infection (MGI Ref ID J:121095)
  • hematopoietic system phenotype
    • abnormal CD4-positive T cell morphology (MGI Ref ID J:121095)
      • number of Il-4 single producing cells is doubled in culture with nonpolarizing conditions (ThN)
      • abnormal T-helper 1 cell morphology (MGI Ref ID J:121095)
        • mutant Th1 cells produce elevated levels of CCL-5
      • increased T-helper 2 cell number (MGI Ref ID J:121095)
        • commitment to Th2 pathway is increased; number of Il-4 producing cells in is increased

Strain of Origin: 129S7/SvEvBrd-Hprt

Strain genetic background: B6.Cg

Strain Development: Original allele targeted on AB2.2 ES cells. Males chimaeras were mated directly to C57BL/6 females. The original allele contained a floxed NEO cassette. To remove the NEO cassette the mice were bred to CMV-Cre deleter strain. Mice were then backcrossed 5 generations onto C57BL/6 genetic background.

Suggested Control Mice:

Wild-type littermates

Research Applications

  • Immunology and Inflammation

Strain Origin

Donor: Allan Bradley, Ph.D., Wellcome Trust Sanger Institute.

Primary Reference:

Rodriguez A, Vigorito E, Clare S, Warren MV, Couttet P, Soond DR, van Dongen S, Grocock RJ, Das PP, Miska EA, Vetrie D, Okkenhaug K, Enright AJ, Dougan G, Turner M, Bradley A. Requirement of bic/microRNA-155 for normal immune function. Science. 2007 Apr 27;316(5824):608-11. (Medline PMID: 17463290)

Colony and Husbandry Information

Special Considerations

The mice are immune-deficient. A Helicobacter free facility is required to avoid spontaneous inflammatory bowel disease in this strain.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color:

The mice held by the MMRRC are 99% black but on rare occasion an albino mouse is found, so we can confirm there is residual heterozygosity at the c locus."

Other:

Breeding

MMRRC Breeding System: Backcross of Sib-mating (once colony is established, it can be maintained homozygous)

Breeding Scheme(s):

  • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Homozygous female x Homozygous male littermate


Generation: N5

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: 4 months 5 months

Average litter size: 6

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
029594-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
029594-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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