Strain Name:
B6.Cg-Mir155tm1.1Brd Tyrc-Brd/Mmmh
Stock Number:
029594-MU
Citation ID:
RRID:MMRRC_029594-MU

Strain Information

Mir155tm1.1Brd
Name: microRNA 155; targeted mutation 1.1, Allan Bradley
Synonyms: bicm2, bicBrd-m2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
Tyrc-Brd
Name: tyrosinase; albino, Allan Bradley
Synonyms: C57BL/6c-, cBrd
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Tyr
Name: tyrosinase
Synonyms: skc35, Oca1
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 22173
Homologene: 30969
Mir155
Name: microRNA 155
Synonyms: Mirn155, mmu-mir-155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
NCBI: 387173
VEGA: 16
Genetic Alterations
A small deletion was created to remove exon 2 of the Bic/miR155 locus. This is a null allele for the locus.
Genotype Determination
ES Cell Line
AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2
Phenotype
Homozygous: Immuno-compromised mice. Defective responses by B/T cells and dendritic cells.

Heterozygous: mice have no phenotype.
Mammalian Phenotype Terms
Allelic Composition: Mir155tm1.1Brd/Mir155tm1.1Brd (Genetic Background: B6.129S7-Mir155tm1.1Brd )

Allelic Composition: Mir155tm1.1Brd/Mir155tm1.1Brd (Genetic Background: B6.Cg-Mir155tm1.1Brd Tyrc-Brd )

Allelic Composition: Tyrc-Brd/Tyrc-Brd (Genetic Background: C57BL/6Brd-Tyrc-Brd )

MeSH Terms
  • 3' Untranslated Regions
  • Animals
  • B-Lymphocytes/immunology
  • Cytokines/biosynthesis
  • Dendritic Cells/immunology
  • Gene Expression Regulation
  • Gene Targeting
  • Homeostasis
  • Immune System/physiology
  • Immunoglobulin G/biosynthesis
  • Lung/pathology
  • Lung Diseases/immunology
  • Lung Diseases/pathology
  • Lymphocyte Activation
  • Mice
  • MicroRNAs/genetics
  • MicroRNAs/physiology
  • Oligonucleotide Array Sequence Analysis
  • Proto-Oncogene Proteins c-maf/genetics
  • Proto-Oncogene Proteins c-maf/physiology
  • Salmonella Infections, Animal/immunology
  • T-Lymphocytes/immunology
  • Th1 Cells/immunology
  • Th2 Cells/immunology
  • Vaccination
Strain Development
Original allele targeted on AB2.2 ES cells. Males chimaeras were mated directly to C57BL/6 females. The original allele contained a floxed NEO cassette. To remove the NEO cassette the mice were bred to CMV-Cre deleter strain. Mice were then backcrossed 5 generations onto C57BL/6 genetic background.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Allan Bradley, Ph.D., Wellcome Trust Sanger Institute.
Primary Reference
Rodriguez A, Vigorito E, Clare S, Warren MV, Couttet P, Soond DR, van Dongen S, Grocock RJ, Das PP, Miska EA, Vetrie D, Okkenhaug K, Enright AJ, Dougan G, Turner M, Bradley A. Requirement of bic/microRNA-155 for normal immune function. Science. 2007 Apr 27;316(5824):608-11. (Medline PMID: 17463290)

Colony and Husbandry Information

The mice are immune-deficient. A Helicobacter free facility is required to avoid spontaneous inflammatory bowel disease in this strain.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color

The mice held by the MMRRC are 99% black but on rare occasion an albino mouse is found, so we can confirm there is residual heterozygosity at the c locus."

MMRRC Breeding System
Backcross of Sib-mating (once colony is established, it can be maintained homozygous)
Breeding Scheme(s)
  • Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Homozygous female x Homozygous male littermate


Generation
N5
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 4 months 5 months
Average litter size
6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
029594-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
029594-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.