Strain Name:
129S6;129P2-Myh7tm1Unc/Mmnc
Stock Number:
029868-UNC
Citation ID:
RRID:MMRRC_029868-UNC

Strain Information

Myh7tm1Unc
Name: myosin, heavy polypeptide 7, cardiac muscle, beta; targeted mutation 1, University of North Carolina
Synonyms: betaMHC-YFP, YFP-betaMHC
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knock-In
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: MYH-beta/slow, Myhc-b, B-MHC, beta-MHC, betaMHC, MyHC-I, Myhcb
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Knock-In
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Genetic Alterations
Coding seqeunce for YFP was knocked-in into beta MHC gene at the translational start site to produce a "knockin-in/fusion" allel that expresses YFP fused to the native beta MHC protein.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    E14 derived from 129P2/OlaHsd
    Phenotype
    Homozygous: All cardiac myocytes at the fetal stage, and a small number of cardiac myocytes at the adult stage express YFP-beta MHC fusion protein.

    Heterozygous: All cardiac myocytes at the fetal stage, and a small number of cardiac myocytes at the adult stage express YFP-beta MHC fusion protein.
    Mammalian Phenotype Terms
    Allelic Composition: Myh7tm1Unc/Myh7+ (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    MeSH Terms
    • Aging/genetics
    • Aging/pathology
    • Animals
    • Animals, Newborn
    • Bacterial Proteins/genetics
    • Cardiomegaly/genetics
    • Cardiomegaly/pathology
    • Cell Enlargement
    • Cell Size
    • Female
    • Fibrosis
    • Gene Expression Regulation, Developmental
    • Luminescent Proteins/genetics
    • Male
    • Mice
    • Mice, Inbred C57BL
    • Mice, Transgenic
    • Myocytes, Cardiac/metabolism
    • Myocytes, Cardiac/pathology
    • Myosin Heavy Chains/genetics
    • Ventricular Myosins/genetics
    Strain Development
    Germline Transimtter mouse (Chimaera) was mated to 129/SvEv female, and transgenic offsprings were mated to 129/SvEv males or females.
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cardiovascular
    • Developmental Biology
    • Models for Human Disease
    • Research Tools
    Donor
    Oliver Smithies, Ph.D., University of North Carolina at Chapel Hill
    Primary Reference
    Pandya K, Kim HS, Smithies O. Fibrosis, not cell size, delineates beta-myosin heavy chain reexpression during cardiac hypertrophy and normal aging in vivo. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16864-9. Epub 2006 Oct 26. (Medline PMID: 17068123)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Breeding Scheme(s)
    Backcross to 129SvEv

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    029868-UNC-SPERM Cryo-preserved spermatozoa $564.00 / $937.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3
    029868-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / $4,837.00
    Non-Profit / For-Profit
    Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.