Allele Symbol: Spry1tm1Jdli
Name: targeted mutation 1, Jonathan D Licht
Alteration at locus: Knockout
Gene Symbol: Spry1
Name: sprouty homolog 1 (Drosophila)
Alteration at locus: Knockout
ES Cell Line: R1 derived from 129X1/SvJ x 129S1/Sv
Homozygous phenotype: black mid-size mouse with black eyes and normal breeding pattern
Heterozygous phenotype: black mid-size mouse with black eyes and normal breeding pattern
Strain of Origin: 129X1/SvJ
Strain genetic background: C57BL/6
Strain Development: Two clones were injected into C57BL6/6 blastocysts.Chimeric males were mated with C57BL/6J females and pups derived from the 129/SvJ ES clones were screened for presence of the targetted allele by PCR of genomic DNA Mice carrying the targetted allele were bred with Flpe deleter mice to remove PGK-Neo cassette.All subsquent analyses was performed in the mixed genetic background (129sv/J:C57BL6).
Suggested Control Mice:
Donor: Dr. Jonathan Licht,M.D., Northwestern University
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.