Strain Detail Sheet

Strain Name    :

B6;129-Spry1tm1Jdli/Mmmh

Stock Number :

029870-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Spry1tm1Jdli
Name: targeted mutation 1, Jonathan D Licht
Alteration at locus: Knockout
Gene Symbol: Spry1
Name: sprouty homolog 1 (Drosophila)
Chromosome: 3
Alteration at locus: Knockout

Genetic Alterations:
An 11.5 kb fragement that contaains all three putative exons, including the third one that encodes the open reading frame was subcloned into p-Bluescript Oligonucleotides containng loxP sequences were inserted into BglII sites flanking the ORF.A PGK-Neo cassettte flanked by frt sequences was inserted into a unique BclI site 3' of the Spry1 ORF to generate targeting construct.

Genotype Determination:

ES Cell Line: R1 derived from 129X1/SvJ x 129S1/Sv

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: black mid-size mouse with black eyes and normal breeding pattern

Heterozygous phenotype: black mid-size mouse with black eyes and normal breeding pattern


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Spry1tm1Jdli/Spry1tm1Jdli
        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • normal phenotype
    • no abnormal phenotype detected (MGI Ref ID J:96485)

Strain of Origin: 129X1/SvJ

Strain genetic background: C57BL/6

Strain Development: Two clones were injected into C57BL6/6 blastocysts.Chimeric males were mated with C57BL/6J females and pups derived from the 129/SvJ ES clones were screened for presence of the targetted allele by PCR of genomic DNA Mice carrying the targetted allele were bred with Flpe deleter mice to remove PGK-Neo cassette.All subsquent analyses was performed in the mixed genetic background (129sv/J:C57BL6).

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Developmental Biology
  • Models for Human Disease
  • Reproduction

Strain Origin

Donor: Dr. Jonathan Licht,M.D., Northwestern University

Primary Reference:

  • Basson MA, Akbulut S, Watson-Johnson J, Simon R, Carroll TJ, Shakya R, Gross I, Martin GR, Lufkin T, McMahon AP, Wilson PD, Costantini FD, Mason IJ, Licht JD. Sprouty1 is a critical regulator of GDNF/RET-mediated kidney induction. Dev Cell. 2005 Feb;8(2):229-39. (Medline PMID: 15691764)
  • Basson MA, Watson-Johnson J, Shakya R, Akbulut S, Hyink D, Costantini FD, Wilson PD, Mason IJ, Licht JD. Branching morphogenesis of the ureteric epithelium during kidney development is coordinated by the opposing functions of GDNF and Sprouty1. Dev Biol. 2006 Nov 15;299(2):466-77. Epub 2006 Aug 25. (Medline PMID: 17022962)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
029870-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
029870-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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