Allele Symbol: Gp5tm1Cgh
Name: targeted mutation 1, Shaun R Coughlin
Alteration at locus: Knockout
Gene Symbol: Gp5
Name: glycoprotein 5 (platelet)
Alteration at locus: Knockout
ES Cell Line: RF8
Strain of Origin: C57B6/129SVjae Mixed
Strain genetic background: C57B6/129SVjae Mixed
Strain Development: Chimeric mouse made with GPV +/- Rf8 ES cells from 129SvJae strain was mated to B6 female to generate GPV heterozygous offspring. Heterozygous crosses generated homozygous offspring. The strain has been maintained by intercrossing these mice. Thus background on average should be 25%129, 75% C57BL/6.
Suggested Control Mice:
Donor: Shaun Coughlin, M.D., Ph.D., University of California San Francisco
Kahn ML, Diacovo TG, Bainton DF, Lanza F, Trejo J, Coughlin SR. Glycoprotein V-deficient platelets have undiminished thrombin responsiveness and Do not exhibit a Bernard-Soulier phenotype. Blood. 1999 Dec 15;94(12):4112-21. (Medline PMID: 10590056)
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.