Strain Name:
B6.Cg-Tg(tetO-Vamp2)1Kdmc/Mmmh
Stock Number:
029874-MU
Citation ID:
RRID:MMRRC_029874-MU

Strain Information

tetOp
Name: tetracycline operon
Type: Gene
Species: Synthetic
Chromosome:
Vamp2
Name: vesicle-associated membrane protein 2
Synonyms: Syb2, synaptobrevin II, Syb-2, sybII, synaptobrevin-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22318
Homologene: 7591
Tg(tetO-Vamp2)1Kdmc
Name: transgene insertion 1, Ken D McCarthy
Synonyms: tetO.SNARE, dominant-negative SNARE, dn-SNARE
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
DNA construct microinjected into fertilized oocytes; transgenic line carrying dominant negative synaptobrevin 2 driven by tetO promoter.
Genotype Determination
Phenotype
Homozygous: not characterized

Hemizygous: not characterized
MeSH Terms
  • Adenosine/metabolism
  • Adenosine A1 Receptor Antagonists
  • Adenosine Triphosphatases/antagonists & inhibitors
  • Adenosine Triphosphate/analogs & derivatives
  • Adenosine Triphosphate/metabolism
  • Adenosine Triphosphate/pharmacology
  • Animals
  • Astrocytes/drug effects
  • Astrocytes/physiology
  • Cells, Cultured
  • Excitatory Postsynaptic Potentials
  • Hippocampus/drug effects
  • Hippocampus/physiology
  • In Vitro Techniques
  • Long-Term Potentiation/drug effects
  • Mice
  • Mice, Transgenic
  • Neuronal Plasticity/drug effects
  • Purinergic P1 Receptor Antagonists
  • Purinergic P2 Receptor Antagonists
  • Receptor, Adenosine A1/metabolism
  • Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
  • Receptors, N-Methyl-D-Aspartate/metabolism
  • Receptors, Purinergic P1/metabolism
  • Receptors, Purinergic P2/metabolism
  • Synapses/physiology
  • Synaptic Transmission/drug effects
  • Xanthines/pharmacology
Strain Development
Founder transgenics were BL6/C3H hybrids. Mice were backcrossed to C57BL6/J.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Neurobiology
  • Research Tools
Donor
Ken D McCarthy, Ph.D., University of North Carolina at Chapel Hill
Primary Reference
Pascual O, Casper KB, Kubera C, Zhang J, Revilla-Sanchez R, Sul JY, Takano H, Moss SJ, McCarthy K, Haydon PG. Astrocytic purinergic signaling coordinates synaptic networks.Science. 2005 Oct 7;310(5745):113-6. (Medline PMID: 16210541)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
029874-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
029874-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.