Strain Name:
B6.129S6-Ptprjtm1Taka/Mmmh
Stock Number:
029875-MU
Citation ID:
RRID:MMRRC_029875-MU

Strain Information

Ptprjtm1Taka
Name: protein tyrosine phosphatase receptor type J; targeted mutation 1, Takamune Takahashi
Synonyms: CD148DeltaCyGFP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knock-In
Ptprj
Name: protein tyrosine phosphatase receptor type J
Synonyms: Byp, CD148, Scc1, DEP-1, Scc-1, RPTPJ
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Knock-In
NCBI: 19271
HGNC: HGNC:9673
Homologene: 2130
Genetic Alterations
An EGFP-coding gene (Clontech) was fused in frame to the murine CD148 gene at the KpnI site. This EGFP insertion results in a catalytically inactive CD148-GFP fusion gene (CD148CyGFP), substituting GFP for the C-terminal 117 amino acid residues containing essential cysteine and the surrounding core motif.
Genotype Determination
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Mice die at midgestation due to vascularization failure marked by growth retardation and disorganized vascular structures.

Heterozygous: The life expectancy, fertility, and gross appearance of the viable heterozygous progeny appeared normal.
Mammalian Phenotype Terms
Allelic Composition: Ptprjtm1Taka/Ptprjtm1Taka (Genetic Background: involves: 129S6/SvEvTac )

Allelic Composition: Ptprjtm1Taka/Ptprj+ (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 )

MeSH Terms
  • Alleles
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Blotting, Northern
  • Blotting, Western
  • Catalysis
  • Catalytic Domain
  • Cell Division
  • Cytoplasm/metabolism
  • DNA-Binding Proteins/metabolism
  • Endothelium, Vascular/cytology
  • Endothelium, Vascular/enzymology
  • Ephrin-B2/metabolism
  • Green Fluorescent Proteins
  • Homozygote
  • Kidney/metabolism
  • Luminescent Proteins/metabolism
  • Mice
  • Microscopy, Electron
  • Models, Genetic
  • Mutation
  • Myocardium/metabolism
  • Platelet Endothelial Cell Adhesion Molecule-1/biosynthesis
  • Precipitin Tests
  • Protein Tyrosine Phosphatases/biosynthesis
  • Protein Tyrosine Phosphatases/genetics
  • Protein Tyrosine Phosphatases/physiology
  • Proto-Oncogene Proteins/metabolism
  • Receptor Protein-Tyrosine Kinases/metabolism
  • Receptor, TIE-2
  • Receptor-Like Protein Tyrosine Phosphatases, Class 3
  • Recombinant Fusion Proteins/metabolism
  • Recombination, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Cell Acute Lymphocytic Leukemia Protein 1
  • Time Factors
  • Transcription Factors/metabolism
  • Tyrosine/metabolism
  • Vascular Endothelial Growth Factor Receptor-2/metabolism
Strain Development
Heterozygous male were backcrossed to C57BL/6J female wildtype for 10 generation.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Hematology
  • Immunology and Inflammation
  • Internal/Organ
  • Models for Human Disease
  • Research Tools
Donor
Takamune Takahashi, M.D., Vanderbilt University Medical Center
Primary Reference
Takahashi T; Takahashi K; St John PL; Fleming PA; Tomemori T; Watanabe T; Abrahamson DR; Drake CJ; Shirasawa T; Daniel TO, A mutant receptor tyrosine phosphatase, CD148, causes defects in vascular development., Mol Cell Biol 2003 Mar;23(5):1817-31 (Medline PMID: 12588999)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
029875-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
029875-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.