Strain Detail Sheet

Strain Name    :

B6;129X1-Als2tm1Cai/Mmmh

Stock Number :

029881-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Als2tm1Cai
Name: targeted mutation 1, Huaibin Cai
Alteration at locus: Knockout
Gene Symbol: Als2
Name: amyotrophic lateral sclerosis 2 (juvenile)
Chromosome: 1
Alteration at locus: Knockout

Genetic Alterations:
A 39 base-pairs BamHI fragment in the exon 3 of the ALS2 gene was deleted and replaced with neomycin gene selection cassette.

Genotype Determination:

ES Cell Line: Not specified derived from B6;129S7

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: ALS2-/- mice are viable, fertile and display no obvious developmental abnormalities. They exhibit age-dependent deficits in motor coordination and learning, and show a higher anxiety response in the open field and elevated plus maze tasks.

Heterozygous phenotype: ALS2+/- mice exhibit a moderate age-dependent deficits in motor learning by repeated Rotarod test.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Als2tm1Cai/Als2tm1Cai
        involves: 129X1/SvJ * C57BL/6J
  • mortality/aging
    • increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:100336)
      • intraperitoneal injection of 35 mg/kg paraquat induced death in about 50% of homozygotes compared to 0% of control mice
      • homozygotes are not as sensitive to paraquat-induced mortality as are Ccstm1Pcw homozygotes
  • growth/size phenotype
    • increased body weight (MGI Ref ID J:100336)
      • body weight is normal in young mice but increased by 18% and 25% at 12 and 20 months of age, respectively, compared to wild-type littermates
  • behavior/neurological phenotype
    • abnormal motor learning (MGI Ref ID J:100336)
      • no significant change in the latency to fall from a rotating rod is seen between 9 and 20 months of age and in repeated trials over multiple days homozygotes do not perform better in the first trials of day compared to their performance in the last trial of the preceding day
    • decreased exploration in new environment (MGI Ref ID J:100336)
      • a decrease in locomotor activity is seen in a novel environment; however, gait, grip, muscle tone, and startle response are all normal and no decrease in spontaneous activity is seen in a Flex-Field apparatus
    • impaired coordination (MGI Ref ID J:100336)
      • at 12 and 20 months of age homozygotes display a decreased latency to fall from a rotating rod compared to wild-type littermates
    • increased anxiety-related response (MGI Ref ID J:100336)
      • homozygotes spend less time in the center of an open field and spend less time and make fewer visits to the open arms of an elevated plus maze
  • homeostasis/metabolism phenotype
    • increased physiological sensitivity to xenobiotic (MGI Ref ID J:100336)
      • increased sensitivity to paraquat-induced in vitro neuronal cell death is seen in homozygous mice compared to wild-type littermates
    • increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:100336)
      • intraperitoneal injection of 35 mg/kg paraquat induced death in about 50% of homozygotes compared to 0% of control mice
      • homozygotes are not as sensitive to paraquat-induced mortality as are Ccstm1Pcw homozygotes
  • nervous system phenotype
    • nervous system phenotype (MGI Ref ID J:100336)
      • no significant changes in peripheral nerve conduction or electromyography and no gross neuropathological abnormalities are seen

Strain of Origin: 129X1/SvJ

Strain genetic background: a hybrid of 129 and C57BL/6J

Strain Development: The male chimeras were mated with C57BL/6J female mice to obtain the ALS2+/- mice. The ALS2+/- mice were intercrossed to obtain the ALS2-/- animals. Later on, ALS2-/- mice were also intercrossed to keep the colony. All ALS2-/- mice are a hybrid of 129X1/SvJ and C57BL/6J strain backgrounds.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cell Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Huaibin Cai, Ph.D., NIH, NIA

Primary Reference:

Cai H, Lin X, Xie C, Laird FM, Lai C, Wen H, Chiang HC, Shim H, Farah MH, Hoke A, Price DL, Wong PC. Loss of ALS2 function is insufficient to trigger motor neuron degeneration in knock-out mice but predisposes neurons to oxidative stress. J Neurosci. 2005 Aug 17;25(33):7567-74. (Medline PMID: 16107644)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
029881-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
029881-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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