Allele Symbol: Als2tm1Cai
Name: targeted mutation 1, Huaibin Cai
Alteration at locus: Knockout
Gene Symbol: Als2
Name: amyotrophic lateral sclerosis 2 (juvenile)
Alteration at locus: Knockout
ES Cell Line: Not specified derived from B6;129S7
Homozygous phenotype: ALS2-/- mice are
viable, fertile and display no obvious developmental abnormalities. They
exhibit age-dependent deficits in motor coordination and learning, and
show a higher anxiety response in the open field and elevated plus maze
Heterozygous phenotype: ALS2+/- mice exhibit a moderate age-dependent deficits in motor learning by repeated Rotarod test.
Strain of Origin: 129X1/SvJ
Strain genetic background: a hybrid of 129 and C57BL/6J
Strain Development: The male chimeras were mated with C57BL/6J female mice to obtain the ALS2+/- mice. The ALS2+/- mice were intercrossed to obtain the ALS2-/- animals. Later on, ALS2-/- mice were also intercrossed to keep the colony. All ALS2-/- mice are a hybrid of 129X1/SvJ and C57BL/6J strain backgrounds.
Suggested Control Mice:
Donor: Huaibin Cai, Ph.D., NIH, NIA
Cai H, Lin X, Xie C, Laird FM, Lai C, Wen H, Chiang HC, Shim H, Farah MH, Hoke A, Price DL, Wong PC. Loss of ALS2 function is insufficient to trigger motor neuron degeneration in knock-out mice but predisposes neurons to oxidative stress. J Neurosci. 2005 Aug 17;25(33):7567-74. (Medline PMID: 16107644)
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.