Strain Name    : B6;129X1-Als2tm1Cai/Mmmh

Stock Number : 029881-MU

Gene Information

(provided by MGI)
Allele Symbol: Als2tm1Cai
Name: targeted mutation 1, Huaibin Cai
Chromosome: 1
Alteration at locus: Knockout
Gene Symbol: Als2
Name: amyotrophic lateral sclerosis 2 (juvenile)
Chromosome: 1
Alteration at locus: Knockout
Genetic Alterations:
A 39 base-pairs BamHI fragment in the exon 3 of the ALS2 gene was deleted and replaced with neomycin gene selection cassette.

Genotype Determination:

ES Cell Line: Not specified derived from B6;129S7

Phenotype

Homozygous phenotype: ALS2-/- mice are viable, fertile and display no obvious developmental abnormalities. They exhibit age-dependent deficits in motor coordination and learning, and show a higher anxiety response in the open field and elevated plus maze tasks.

Heterozygous phenotype: ALS2+/- mice exhibit a moderate age-dependent deficits in motor learning by repeated Rotarod test.

Mammalian Phenotype Terms:
Allelic Composition: Als2tm1Cai/Als2tm1Cai (Genetic Background: involves: 129X1/SvJ * C57BL/6J )

Strain of Origin: 129X1/SvJ

Strain genetic background: a hybrid of 129 and C57BL/6J

Strain Development: The male chimeras were mated with C57BL/6J female mice to obtain the ALS2+/- mice. The ALS2+/- mice were intercrossed to obtain the ALS2-/- animals. Later on, ALS2-/- mice were also intercrossed to keep the colony. All ALS2-/- mice are a hybrid of 129X1/SvJ and C57BL/6J strain backgrounds.

Suggested Control Mice:

  • Wildtype littermates

  • Cell Biology
  • Models for Human Disease
  • Neurobiology

Donor: Huaibin Cai, Ph.D., NIH, NIA

Primary Reference:

Cai H, Lin X, Xie C, Laird FM, Lai C, Wen H, Chiang HC, Shim H, Farah MH, Hoke A, Price DL, Wong PC. Loss of ALS2 function is insufficient to trigger motor neuron degeneration in knock-out mice but predisposes neurons to oxidative stress. J Neurosci. 2005 Aug 17;25(33):7567-74. (Medline PMID: 16107644)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
029881-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
029881-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.