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Strain Name:
B6.129S4(Cg)-Dnmt3btm5.1Enl/Mmnc
Stock Number:
029887-UNC
Citation ID:
RRID:MMRRC_029887-UNC

Strain Information

Dnmt3btm5.1Enl
Name: DNA methyltransferase 3B; targeted mutation 5.1, En Li
Synonyms: Dnmt3bfl, Dnmt3bflox, Dnmt3b2lox, Dnmt3b2loxP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Targeted Mutation
Dnmt3b
Name: DNA methyltransferase 3B
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Targeted Mutation
NCBI: 13436
HGNC: HGNC:2979
Homologene: 56000
Genetic Alterations
A loxP-flanked PGK-neo cassette was inserted in the intron upstream of exon 19 and a third loxP site in the intron between exons 19 and 20. Cre-mediated recombination deleted the selection cassette in the germline, leaving loxP sites upstream and downstream of exon 19. When mice bearing the present mutation are crossed to Cre recombinase expressing mice, deletion of the floxed DNA segment leads to out-of-frame splicing of the mRNAs encoding all active isoforms of the protein in the tissues where Cre is expressed.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    J1 derived from 129S4/SvJae
    Phenotype

    Homozygous: normal. 

    Heterozygous: normal.  

    Cre-mediated deletion of catalytic motifs produces functionally null alleles

    MeSH Terms
    • Animals
    • Cell Line, Transformed
    • Cells, Cultured
    • Chromosomal Instability/physiology
    • DNA/genetics
    • DNA/metabolism
    • DNA (Cytosine-5-)-Methyltransferases/antagonists & inhibitors
    • DNA (Cytosine-5-)-Methyltransferases/deficiency
    • DNA (Cytosine-5-)-Methyltransferases/genetics
    • DNA (Cytosine-5-)-Methyltransferases/metabolism
    • DNA Methylation
    • Fibroblasts/enzymology
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    Strain Development
    Original strain was backcrossed with C57BL/6 for multiple generations.
    Suggested Control Mice

    Wild-type littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Developmental Biology
    • Immunology and Inflammation
    • Models for Human Disease
    Donor
    En Li, Ph.D., Novartis Institutes for Biomedical Research
    Primary Reference
    Dodge JE, Okano M, Dick F, Tsujimoto N, Chen T, Wang S, Ueda Y, Dyson N, Li E. Inactivation of Dnmt3b in mouse embryonic fibroblasts results in DNA hypomethylation, chromosomal instability, and spontaneous immortalization. J Biol Chem. 2005 May 6;280(18):17986-91. Epub 2005 Mar 8. (Medline PMID: 15757890)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    029887-UNC-EMBRYO Cryo-preserved embryos $1,488.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    029887-UNC-SPERM Cryo-preserved spermatozoa $520.00 / Non-Profit Aliquot Approximate quantity3
    029887-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.