Strain Name:
STOCK Mfn1tm2Dcc/Mmucd
Stock Number:
029901-UCD
Citation ID:
RRID:MMRRC_029901-UCD
Other Names:
Mfn1loxP, STOCK Mfn1tm2Dcc/Mmcd

Strain Information

Mfn1tm2Dcc
Name: mitofusin 1; targeted mutation 2, David C Chan
Synonyms: Mfn1loxP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Targeted Mutation
Mfn1
Name: mitofusin 1
Synonyms: 6330416C07Rik, 2310002F04Rik, D3Ertd265e, HR2
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Targeted Mutation
NCBI: 67414
Homologene: 11481
Genetic Alterations
In this strain, an FRT-flanked neo cassette followed by two loxP sites flank the Mfn1 exon 4 encoding the canonical G-1 GTPase motif replaced the endogenous locus. The neo cassette was removed by crossing with FLP recombinase expressing mice.
Genotype Determination
ES Cell Line
Unspecified
Phenotype
Homozygous: Conditional alleles behave as wild-type
Heterozygous: Not distinguishable from wild-type.
Expected cre excised phenotype:when excised, homozygotes are embryonic lethal: Mfn1 at ~e11.5, Mfn2 at ~e10.5.
MeSH Terms
  • Animals
  • Cerebellum/enzymology
  • Cerebellum/pathology
  • Cerebellum/ultrastructure
  • GTP Phosphohydrolases/genetics
  • Intracellular Membranes/enzymology
  • Intracellular Membranes/physiology
  • Membrane Fusion/genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mitochondria/genetics
  • Mitochondria/ultrastructure
  • Neurodegenerative Diseases/genetics
  • Neurodegenerative Diseases/pathology
  • Neurodegenerative Diseases/prevention & control
  • Purkinje Cells/enzymology
  • Purkinje Cells/pathology
  • Cell Movement/genetics
  • Cells, Cultured
  • Cytoskeletal Proteins/genetics
  • Embryo Loss/genetics
  • Embryo, Mammalian/cytology
  • Embryo, Mammalian/embryology
  • Embryo, Mammalian/metabolism
  • Embryonic and Fetal Development/genetics
  • Female
  • Fetus
  • GTP Phosphohydrolases
  • Gene Expression Regulation, Developmental/physiology
  • Genes, Lethal/genetics
  • Intracellular Membranes/metabolism
  • Intracellular Membranes/pathology
  • Macromolecular Substances
  • Male
  • Membrane Potentials/genetics
  • Membrane Proteins/deficiency
  • Membrane Proteins/genetics
  • Membrane Transport Proteins
  • Mitochondria/metabolism
  • Mitochondria/pathology
  • Mitochondrial Membrane Transport Proteins
  • Mitochondrial Proteins/deficiency
  • Mitochondrial Proteins/genetics
  • Placenta/abnormalities
  • Placenta/metabolism
  • Placenta/physiopathology
  • Trophoblasts/metabolism
  • Trophoblasts/pathology
  • Utrophin
Strain Development
Correctly targeted 129 ES cells were implanted into C57BL/6 blastocysts. Resulting chimeras were mated to Black Swiss outbred mice. Agouti offspring were mated with FlpER mice (129 background). Neo-excised offspring were mated to Black Swiss offspring w/o Flp crossed to generate homozygous lines maintained as random intrastrain crosses for ~12 generations. The strain is now maintained with Mfn1loxP strain crossed ) to wild-type of 129/Black Swiss mixed background, and then homozygosed again.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Neurobiology
  • Reproduction
Donor
David Chan, M.D., Ph.D., California Institute of Technology
Primary Reference
  • Chen H, McCaffery JM, Chan DC. Mitochondrial fusion protects against neurodegeneration in the cerebellum. Cell. 2007 Aug 10;130(3):548-62. (Medline PMID: 17693261)
  • Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J Cell Biol. 2003 Jan 20;160(2):189-200. Epub 2003 Jan 13. (Medline PMID: 12527753)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black or agouti
MMRRC Breeding System
Intra-strain Random Mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male from the colony or reciprocal mating
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
029901-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
029901-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
029901-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.