Strain Detail Sheet

Strain Name    :

STOCK Mfn1tm2Dcc/Mmucd

Stock Number :

029901-UCD

Other Names   :

Mfn1loxP, STOCK Mfn1tm2Dcc/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mfn1tm2Dcc
Name: targeted mutation 2, David C Chan
Alteration at locus: Targeted Mutation
Gene Symbol: Mfn1
Name: mitofusin 1
Chromosome: 3
Alteration at locus: Targeted Mutation

Genetic Alterations:
LoxP sites were inserted to flank the exon containing the conserved G1-GTPase motif of each gene

Genotype Determination:

ES Cell Line: Not specified derived from 129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous: Conditional alleles behave as wild-type
Heterozygous: Not distinguishable from wild-type.
Expected cre excised phenotype:when excised, homozygotes are embryonic lethal: Mfn1 at ~e11.5, Mfn2 at ~e10.5.

Strain of Origin: 129

Strain genetic background: mixed background of 129 and Black Swiss

Strain Development: Correctly targeted 129 ES cells were implanted into C57BL/6 blastocysts.  Resulting chimeras were mated to Black Swiss outbred mice. Agouti offspring were mated with FlpER mice (129 background). Neo-excised offspring were mated to Black Swiss offspring w/o Flp crossed to generate homozygous lines maintained as random intrastrain crosses for ~12 generations. The strain is now maintained with Mfn1loxP strain crossed ) to wild-type of 129/Black Swiss mixed background, and then homozygosed again.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Neurobiology
  • Reproduction

Strain Origin

Donor: David Chan, M.D., Ph.D., California Institute of Technology

Primary Reference:

  • Chen H, McCaffery JM, Chan DC. Mitochondrial fusion protects against neurodegeneration in the cerebellum. Cell. 2007 Aug 10;130(3):548-62. (Medline PMID: 17693261)
  • Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J Cell Biol. 2003 Jan 20;160(2):189-200. Epub 2003 Jan 13. (Medline PMID: 12527753)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Black or agouti

Other:

Breeding

MMRRC Breeding System: Intra-strain Random Mating

Breeding Scheme(s):

  • Wild-type female x Heterozygous male from the colony or reciprocal mating

Generation:

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: Undetermined Undetermined

Average litter size: 8

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
029901-UCD-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
029901-UCD-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
029901-UCD-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/24/2014