Strain Name:
A.129P2(B6)-Esr2tm1Unc/Mmmh
Stock Number:
029908-MU
Citation ID:
RRID:MMRRC_029908-MU

Strain Information

Esr2tm1Unc
Name: estrogen receptor 2 (beta); targeted mutation 1, University of North Carolina
Synonyms: BERKO, ERbetaKO, beta-ERKO, ERbeta-, betaERKO, Erb-, BERKOChapel Hill
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Esr2
Name: estrogen receptor 2 (beta)
Synonyms: Estrb, oestrogen receptor beta, ER beta, ERbeta
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knockout
NCBI: 13983
HGNC: HGNC:3468
Homologene: 1100
Genotype Determination
ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd
Phenotype
Homozygous: The phenotype of homozygous A.Cg-Esr1tmUNC and A.Cg-Esr2tmUNC mice has not been fully characterized. Female Esr1 knockout mice have small underdeveloped uteri and small thymi when compared to wild-type controls indicative of loss of estrogen influence on reproductive tract development. Donor's research focused on the mucosal immune response to an intestinal bacterial colonizer (Helicobacter hepaticus). Preliminary data suggests that Esr2 knockouts are less susceptible to disease than Esr1 knockouts and wildtype and heterozygous controls.

Heterozygous: Heterozygous mice appear to be similar phenotypically to wild type mice reproductively, behaviorally and in their response to H. hepaticus.
Mammalian Phenotype Terms
Allelic Composition: Esr2tm1Unc/Esr2tm1Unc (Genetic Background: B6.129P2-Esr2tm1Unc )

Allelic Composition: Esr2tm1Unc/Esr2tm1Unc (Genetic Background: involves: 129P2/OlaHsd )

Allelic Composition: Esr2tm1Unc/Esr2tm1Unc (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Allelic Composition: Esr2tm1Unc/Esr2tm1Unc (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

MeSH Terms
  • Animals
  • Base Sequence
  • DNA Primers/chemistry
  • Estrogens/metabolism
  • Female
  • Fertility/physiology
  • Genes
  • Male
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Ovary/embryology
  • Phenotype
  • Receptors, Estrogen/physiology
  • Reproduction
  • Restriction Mapping
  • Sex Differentiation/physiology
  • Uterus/embryology
  • Estrogen Receptor alpha
  • Estrogen Receptor beta
  • Exons
  • Genes, Reporter
  • Infertility, Female/genetics
  • Infertility, Male/genetics
  • Ovary/cytology
  • Ovary/metabolism
  • Ovary/pathology
  • Receptors, Estrogen/deficiency
  • Receptors, Estrogen/genetics
  • Recombination, Genetic
  • Reproduction/genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stem Cells/physiology
  • Transfection
Strain Development
Esr1 (ER alpha receptor gene) and Esr2 (ER beta receptor gene) mutant mice on a C57BL/6 background were obtained from Dr. Dennis Lubahn, University of Missouri as part of an ongoing collaboration. Both mutants were backcrossed to A/JCr mice to generate congenic mice. Backcross progeny were genotyped using microsatellite probes and mice with either the highest percent of A/J markers fixed to homozygosity or those with linked markers fixed to A/J homozygosity were selected for subsequent backcross generations (speed congenics approach). After 5 generations, all markers for both mutants were fixed to homozygosity for A/J alleles. While the majority of backcross pairings involved male congenics bred to female A/J, one generation was performed with female congenics and male A/Js to fix the Y chromosome. The colony was backcrossed for an additional 3-4 generations (8-9 backcrosses total). At that time, heterozygous mice for each mutation were bred together to generate double heterozygous mice. For reanimation, breeding of double heterozygous mice would result in the ability to generate both Esr1 mutants, Esr2 mutants and double knockouts as well as heterozygotes for subsequent colony production. On the C57BL/6 background, homozygous mutants are infertile; donor did not try to breed homozygous mutants of the A/J background.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Cardiovascular
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
Donor
Craig Franklin, DVM, University of Missouri
Primary Reference
  • Lubahn DB, Moyer JS, Golding TS, Couse JF, Korach KS, Smithies O. Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen receptor gene. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11162-6. (Medline PMID: 8248223)
  • Krege JH, Hodgin JB, Couse JF, Enmark E, Warner M, Mahler JF, Sar M, Korach KS, Gustafsson JA, Smithies O. Generation and reproductive phenotypes of mice lacking estrogen receptor beta. Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15677-82. (Medline PMID: 9861029)

Colony and Husbandry Information

On the C57BL/6 background, homozygous mutants are infertile; donor did not try to breed homozygous mutants of the A/J background.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
029908-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
029908-MU-RESUS Litter recovered from cryo-archive $2,697.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.