Strain Name:
Stock Number:
Citation ID:
Other Names:
TRAM knockout
Major Collection:

Strain Information

Name: toll-like receptor adaptor molecule 2; targeted mutation 1, Bruce Beutler
Synonyms: Tram-, TRAM KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Knockout
Name: toll-like receptor adaptor molecule 2
Synonyms: TRAM, Tirp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Knockout
NCBI: 225471
Homologene: 11014
Genetic Alterations
Exon 3, which encompasses the entire coding region of TRAM, was replaced with a neo cassette.
Genotype Determination
ES Cell Line
Not specified
Homozygous: Tram knockout macrophages stimulated in vitro with LPS produce no type I IFN, and reduced amounts of TNF-alpha.

Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Ticam2tm1Btlr/Ticam2tm1Btlr (Genetic Background: C57BL/6J-Ticam2tm1Btlr )

  • immune system
MeSH Terms
  • Adaptor Proteins, Vesicular Transport/immunology
  • Animals
  • Cell Line
  • Cell Survival
  • Dendritic Cells/immunology
  • Female
  • Gene Expression Regulation
  • Humans
  • Interferon Regulatory Factor-7/biosynthesis
  • Interferon Type I/biosynthesis
  • Macrophages, Peritoneal/virology
  • Male
  • Membrane Glycoproteins/immunology
  • Membrane Transport Proteins/immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Models, Animal
  • Myelin Proteins/immunology
  • Myelin and Lymphocyte-Associated Proteolipid Proteins
  • Myeloid Differentiation Factor 88/immunology
  • Proteolipids/immunology
  • RNA, Messenger/biosynthesis
  • Receptors, Interleukin/immunology
  • Signal Transduction
  • Toll-Like Receptor 4/immunology
  • Toll-Like Receptor 4/metabolism
  • Vesicular stomatitis Indiana virus/immunology
  • Viral Envelope Proteins/immunology
Strain Development
The original mutant was created by targeted knockout of Tram in C57BL/6J background. The mutant strain has been maintained by homozygous breeding with the C57BL/6J background.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact Older strains may not have this information.
  • Immunology and Inflammation
  • Models for Human Disease
Bruce Beutler, M.D., Scripps Research Institute
Primary Reference

Georgel P, Jiang Z, Kunz S, Janssen E, Mols J, Hoebe K, Bahram S, Oldstone MB, Beutler B. Vesicular stomatitis virus glycoprotein G activates a specific antiviral Toll-like receptor 4-dependent pathway. Virology. 2007 Jun 5;362(2):304-13. Epub 2007 Feb 12. (Medline PMID: 17292937)

For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030018-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030018-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
030018-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.