Strain Name    : C57BL/6J-Gimap5m1Btlr/Mmucd

Stock Number : 030019-UCD

Other Names   : Sphinx, C57BL/6J-Gimap5m1Btlr/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Gimap5m1Btlr
Name: mutation 1, Bruce Beutler
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Gimap5
Name: GTPase, IMAP family member 5
Chromosome: 6
Alteration at locus: Chemically Induced
Genetic Alterations:
The mutation is an ENU-induced G to T transversion at position 352 in exon 3 of the Gimap5 gene.

Genotype Determination:

ES Cell Line: Not applicable

Additional mutations found on this strain:

AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00899:Gimap5 APN 6 48753173 missense probably benign 0.01
IGL01936:Gimap5 APN 6 48753065 missense probably benign 0.07
IGL01995:Gimap5 APN 6 48752793 missense possibly damaging 0.86
IGL02371:Gimap5 APN 6 48753003 missense probably damaging 0.98
R0321:Gimap5 UTSW 6 48750515 splice donor site probably benign
R1480:Gimap5 UTSW 6 48753030 missense probably damaging 1.00
R1655:Gimap5 UTSW 6 48753176 nonsense probably null
R1761:Gimap5 UTSW 6 48753261 missense probably damaging 1.00

Phenotype

Homozygous phenotype: Sphinx homozygous mice do not have CD8+ T cells or NK cells and there is a progressive decline in B cell and CD4+ T cell numbers. After 6 weeks of age, homozygotes develop progressive normocytic anemia (with increased variation in RBC width and thrombocytopenia), diarrhea and rapid weight loss weight with death between 8 and 12 weeks. Livers acquire an abnormal morphology, with hyperplasia and nodules resembling tumors.

Heterozygous phenotype: Wild-type.

Mammalian Phenotype Terms:
Allelic Composition: Gimap5m1Btlr/Gimap5m1Btlr (Genetic Background: either: (involves: BALB/cJ * C57BL/6J) or (involves: C3H/HeN * C57BL/6J) or (C57BL/6J * DBA/2J) or (involves: C57BL/6J * FVB/NJ) or (involves: C57BL/6J * NOD/ShiLtJ) )

Allelic Composition: Gimap5m1Btlr/Gimap5m1Btlr (Genetic Background: involves: C57BL/6J )

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: The original mutant mouse was created in C57BL/6J background by ENU mutagenesis. The mutant strain has been maintained by backcrossing and subsequent heterozygote breeding with the same C57BL/6J background.

Suggested Control Mice: Wild-type littermates

  • Apoptosis
  • Cancer
  • Developmental biology
  • Diabetes/obesity
  • Hematology
  • Immunology and inflammation
  • Models for human disease

Donor: Bruce Beutler, M.D., The Scripps Research Institute, La Jolla, CA

Primary Reference:

  • Barnes MJ, Aksoylar H, Krebs P, Bourdeau T, Arnold CN, Xia Y, Khovananth K, Engel I, Sovath S, Lampe K, Laws E, Saunders A, Butcher GW, Kronenberg M, Steinbrecher K, Hildeman D, Grimes HL, Beutler B, Hoebe K. Loss of T cell and B cell quiescence precedes the onset of microbial flora-dependent wasting disease and intestinal inflammation in Gimap5-deficient mice. J Immunol. 2010 Apr 1;184(7):3743-54. (Medline PMID: 20190135)
  • For additional information, please see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Homozygotes show post-weaning lethality with a life expectancy of 14 weeks.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: Black

Other:

MMRRC Breeding System: Sib-mated

Breeding Scheme(s):

  • Heterozygous female x Heterozygous male

Generation:

Overall Breeding Performance: Good (heterozygotes)

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: Unknown Unknown

Average litter size: 4-6

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
030019-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030019-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030019-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.