Strain Name:
STOCK Gnat1tm1Clma Tg(Rho-Gnat1*G38D)1Noa/Mmmh
Stock Number:
030021-MU
Citation ID:
RRID:MMRRC_030021-MU

Strain Information

Gnat1tm1Clma
Name: G protein subunit alpha transducin 1; targeted mutation 1, C L Makino
Synonyms: Gnat1a-, alpha-t-rod, Gnat1tm1Jl, transducin alpha-, trans-, Tralpha-, Gnat1-, Tr-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Gnat1
Name: G protein subunit alpha transducin 1
Synonyms: Ird1, Tralpha, Gnat-1, Ird2, transducin, irdr
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Knockout
NCBI: 14685
HGNC: HGNC:4393
Homologene: 20084
Rho
Name: rhodopsin
Synonyms: LWS opsin, Noerg1, Ops, Opn2, Red Opsin, L opsin, Long Wavelength Sensitive opsin, Rod Opsin, RP4, opsin 2
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knockout
NCBI: 212541
Homologene: 68068
Tg(Rho-Gnat1*G38D)1Noa
Name: transgene insertion 1, Nikolai Artemyev
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The G38D mutation was introduced into exon 1 (NM_008140.3:c113G>A, predicting p.Gly38Asp, see LUMC Mutalyzer). Furthermore, an epitope for recognition by commercially available antibodies (Glu-Glu) was introduced via NM_008140.3:c.485G>A and c.490G>A (predicting p.Gly162Glu and p.Tyr164Met, respectively).
Genotype Determination
ES Cell Line
W9.5/W95 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Phenotype
Homozygous: G38D has reduced affinity for PDE6gamma. GTPase activity and impaired effector (PDE6) activation by the mutant. Flash responses from individual G38D/T
Mammalian Phenotype Terms
Allelic Composition: Gnat1tm1Clma/Gnat1tm1Clma (Genetic Background: involves: 129S1/Sv )

Allelic Composition: Gnat1tm1Clma/Gnat1tm1Clma (Genetic Background: involves: 129S1/Sv * BALB/c )

MeSH Terms
  • Animals
  • Blotting, Western/methods
  • Dark Adaptation/physiology
  • Disease Models, Animal
  • Electroretinography/methods
  • Eye Proteins/metabolism
  • Gene Expression/physiology
  • Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
  • Immunohistochemistry/methods
  • Membrane Potentials/drug effects
  • Membrane Potentials/physiology
  • Membrane Potentials/radiation effects
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Night Blindness/genetics
  • Night Blindness/pathology
  • Night Blindness/physiopathology
  • Photic Stimulation/methods
  • Retina/metabolism
  • Retina/pathology
  • Retina/physiopathology
  • Retinal Rod Photoreceptor Cells/physiopathology
  • Sensory Thresholds/physiology
  • Transducin/genetics
  • Vision, Ocular/genetics
  • Base Sequence
  • DNA Primers
  • Mice, Knockout
  • Retinal Rod Photoreceptor Cells/metabolism
  • Sequence Deletion
  • Vision, Ocular
Strain Development
The transgenic mouse was originally created on a B6;SJL mixed background. Transgenic founders were crossed with C57BL/6. Then, transgenic offspring were crossed with transducin knockout mice (C;129S1 background) for about 12 generations.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Models for Human Disease
  • Research Tools
Donor
Nikolai Artemyev, Ph.D., University of Iowa
Primary Reference

Moussaif M, Rubin WW, Kerov V, Reh R, Chen D, Lem J, Chen CK, Hurley JB, Burns ME, Artemyev NO. Phototransduction in a transgenic mouse model of Nougaret night blindness. J Neurosci. 2006 Jun 21;26(25):6863-72. (Medline PMID: 16793893)

Calvert PD, Krasnoperova NV, Lyubarsky AL, Isayama T, Nicoló M, Kosaras B, Wong G, Gannon KS, Margolskee RF, Sidman RL, Pugh EN Jr, Makino CL, Lem J. Phototransduction in transgenic mice after targeted deletion of the rod transducin alpha -subunit. Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13913-8. Erratum in: Proc Natl Acad Sci U S A 2001 Aug 28;98(18):10515. (Medline PMID: 11095744)

Colony and Husbandry Information

Mice are homozygous for Gnat1 KO mutation and are Gnat1 transgene positive (zygosity cannot be distinguished between hemizygous and homozygous for the transgene). Donor bred transgenic mice with transducin knockout animals, but they can be bred by inbreeding. Some of the offspring will be knockout that can be used for further breeding.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color

Brown

Eye
Dark brown
MMRRC Breeding System
Backcross
Generation
N12 (Gnat1tm1Clma line from PMID:11095744)
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 8 months
Bred to Homozygosity
Yes
Average litter size
9
Recommended wean age
3 weeks
Average Pups Weaned
9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030021-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
030021-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.