Name: targeted mutation 1, Cynthia C Morton
Alteration at locus: Knock-In
Name: coagulation factor C homolog (Limulus polyphemus)
Alteration at locus: Knock-In
ES Cell Line: J1 derived from 129S4/SvJae
Homozygous phenotype: Vestibular (inner
ear, balance) deficits as measured by vestibular evoked potentials.
Slightly elevated auditory brain stem repsonse thresholds. Degeneration of
fibrocytes in inner ear with possible reduced cochlear function.
Heterozygous phenotype: Heterozygotes appear to have vestibular deficits as measure by vestibular evoked potentials.
Strain of Origin: 129SvJ and C57BL/6
Strain genetic background: CBA/CaJ
Strain Development: The original mutant mouse was crossed to EIIa/cre expressing mice (C57BL/6 background). Subsequent to this cross, 11 crosses have been performed to the CBA/CaJ strain.
Suggested Control Mice:
Donor: Cynthia C. Morton, Ph.D., Brigham and Women's Hospital Harvard Medical School
Robertson NG, Jones SM, Sivakumaran TA, Giersch AB, Jurado SA, Call LM, Miller CE, Maison SF, Liberman MC, Morton CC. A targeted Coch missense mutation: a knock-in mouse model for DFNA9 late-onset hearing loss and vestibular dysfunction. Hum Mol Genet. 2008 Aug 12. [Epub ahead of print] (Medline PMID: 18697796)
Donor suggests: Coss into CBA/CaJ which have good hearing at late ages, in order to prevent confounding analysis of hearing at advanced ages.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.