Strain Name:
CBACa.129S4(Cg)-Cochtm1Mrtn/Mmnc
Stock Number:
030022-UNC
Citation ID:
RRID:MMRRC_030022-UNC
Other Names:
Coagulation factor C homology

Strain Information

Cochtm1Mrtn
Name: cochlin; targeted mutation 1, Cynthia C Morton
Synonyms: CochG88E
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knock-In
Coch
Name: cochlin
Synonyms: Coch-5B2, D12H14S564E
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knock-In
NCBI: 12810
VEGA: 12
HGNC: HGNC:2180
Homologene: 20868
Genetic Alterations
Neo cassette was inserted into intron 5, but subsequently removed, with one remaining lox site (and some polylinker sequence). A one base pair mutation in exon 5, resulting in G88E amino acid change was introduced.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    J1 derived from 129S4/SvJae
    Phenotype
    Homozygous: Vestibular (inner ear, balance) deficits as measured by vestibular evoked potentials. Slightly elevated auditory brain stem repsonse thresholds. Degeneration of fibrocytes in inner ear with possible reduced cochlear function.

    Heterozygous: Heterozygotes appear to have vestibular deficits as measure by vestibular evoked potentials.
    Mammalian Phenotype Terms
    Allelic Composition: Cochtm1Mrtn/Cochtm1Mrtn (Genetic Background: CBA/Ca.129S4-Cochtm1Mrtn )

    Allelic Composition: Cochtm1Mrtn/Coch+ (Genetic Background: CBA/Ca.129S4-Cochtm1Mrtn )

    MeSH Terms
    • Animals
    • Cochlear Duct/pathology
    • Disease Models, Animal
    • Evoked Potentials, Auditory, Brain Stem
    • Extracellular Matrix Proteins
    • Gene Knock-In Techniques
    • Hearing Loss/genetics
    • Mice
    • Mice, Inbred C57BL
    • Mutation, Missense
    • Proteins/genetics
    • Vestibular Diseases/genetics
    • Vestibular Function Tests
    Strain Development
    The original mutant mouse was crossed to EIIa/cre expressing mice (C57BL/6 background). Subsequent to this cross, 11 crosses have been performed to the CBA/CaJ strain.
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Models for Human Disease
    • Sensorineural
    • Hearing and Balance
    Donor
    Cynthia C. Morton, Ph.D., Brigham and Women's Hospital/Harvard Medical School
    Primary Reference
    Robertson NG, Jones SM, Sivakumaran TA, Giersch AB, Jurado SA, Call LM, Miller CE, Maison SF, Liberman MC, Morton CC. A targeted Coch missense mutation: a knock-in mouse model for DFNA9 late-onset hearing loss and vestibular dysfunction. Hum Mol Genet. 2008 Aug 12. 2008 Nov 1;17(21):3426-34. Epub 2008 Aug 12. (Medline PMID: 18697796)

    Colony and Husbandry Information

    Donor suggests: Coss into CBA/CaJ which have good hearing at late ages, in order to prevent confounding analysis of hearing at advanced ages.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    030022-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    030022-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.