Strain Name    : B6;129S-Sphk1tm2Cgh/Mmucd

Stock Number : 030038-UCD

Other Names   : msphk1 conditional, B6;129S-Sphk1tm2Cgh/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Sphk1tm2Cgh
Name: targeted mutation 2, Shaun R Coughlin
Chromosome: 11
Alteration at locus: Knockout
Gene Symbol: Sphk1
Name: sphingosine kinase 1
Chromosome: 11
Alteration at locus: Knockout
Genetic Alterations:
Lox-P frt-Neo-frt cassette was inserted downstream of exon 2 and an additional loxP sequence was inserted downstream of exon 6. The neo cassette was removed by breeding to the FlpE Tg (N10 C57BL/6) mouse line, leaving exons 3-6 floxed. The resulting Msphk1 allele is floxed, to allow excision only upon introduction of the cre recombinase.

Genotype Determination:

ES Cell Line: RF8 derived from B6;129S

Phenotype

Homozygous phenotype: No aberrant abnormalities, reduced levels of plasma S1P.

Heterozygous phenotype: None

Mammalian Phenotype Terms:

Strain of Origin: 129S4/SvJae

Strain genetic background: B6;129S4

Strain Development: Targeted ES cells were injected into C57BL/6 blastocysts, and chimeric maleoffspring were mated to C57BL/6 females. Mice positive for germlinetransmission were mated to C57BL/6 N10 FlpE transgenic mice to remove the neo. Heterozygous matings were set up to generate Sphk1f/f mice. Sphk1f/f mice were derived from three different ES clones.

Suggested Control Mice: Wild-type littermates

  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Immunology and Inflammation

Donor: Shaun Coughlin, M.D., Ph.D., University of California San Francisco

Primary Reference:

Pappu R, Schwab SR, Cornelissen I, Pereira JP, Regard JB, Xu Y, Camerer E, Zheng YW, Huang Y, Cyster JG, Coughlin SR. Promotion of lymphocyte egress into blood and lymph by distinct sources of sphingosine-1-phosphate. Science. 2007 Apr 13;316(5822):295-8. Epub 2007 Mar 15. (Medline PMID: 17363629)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color:

Other:

MMRRC Breeding System: Backcross or Sib-mating

Breeding Scheme(s):

  • C57BL/6 female x Heterozygous male or reciprocal
  • Heterozygous female x Heterozygous male 

Generation: Unknown

Overall Breeding Performance: Good

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Average litter size: 6

Recommended wean age: 3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
030038-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030038-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030038-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.