Strain Name    : 129-Htr1btm1Rhn/Mmnc

Stock Number : 030040-UNC

Gene Information

(provided by MGI)
Allele Symbol: Htr1btm1Rhn
Name: targeted mutation 1, Rene Hen
Alteration at locus: Knockout
Gene Symbol: Htr1b
Name: 5-hydroxytryptamine (serotonin) receptor 1B
Chromosome: 9
Alteration at locus: Knockout
Genetic Alterations:
a neo cassette was inserted into exon 1.

Genotype Determination:

ES Cell Line: D3 derived from 129S2/SvPas

Phenotype

Homozygous phenotype: aggressive

Heterozygous phenotype: almost normal


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Htr1btm1Rhn/Htr1b+
        involves: 129S2/SvPas
  • skeleton phenotype
    • abnormal bone ossification (MGI Ref ID J:146078)
      • increased bone formation at 1 and 3 months of age
    • increased bone mass (MGI Ref ID J:146078)
      • at 1 and 3 months of age
    • increased osteoblast cell number (MGI Ref ID J:146078)
      • at 1 and 3 months of age
Htr1btm1Rhn/Htr1btm1Rhn
        involves: 129S2/SvPas
  • behavior/neurological phenotype
    • abnormal locomotor activation (MGI Ref ID J:96221)
      • higher swimming speed
    • abnormal spatial learning (MGI Ref ID J:96221)
      • long-term spatial learning is improved in aged mice
      • attenuation of age related decline in learning
      • less effect on short-term spatial learning
    • decreased anxiety-related response (MGI Ref ID J:96656)
      • observed as reduced burying behavior
    • decreased startle reflex (MGI Ref ID J:96460)
      • reduced reactivity and less readily sensitized
    • increased aggression towards males (MGI Ref ID J:20209)
      • both frequency and intensity of aggression increased
      • latency to aggressive attacks reduced
      • only results with males reported
    • increased drinking be havior (MGI Ref ID J:96318)
      • drank more than sex matched controls
  • growth/size phenotype
  • reproductive system phenotype
    • small testis (MGI Ref ID J:96318)
  • skeleton phenotype
    • abnormal bone ossification (MGI Ref ID J:146078)
      • increased bone formation at 1 and 3 months of age
    • abnormal osteoblast physiology (MGI Ref ID J:146078)
      • increased osteoblast proliferation
    • increased bone mass (MGI Ref ID J:146078)
      • at 1 and 3 months of age
    • increased osteoblast cell number (MGI Ref ID J:146078)
      • at 1 and 3 months of age
  • endocrine/exocrine gland phenotype
    • small testis (MGI Ref ID J:96318)
  • hearing/vestibular/ear phenotype
    • decreased startle reflex (MGI Ref ID J:96460)
      • reduced reactivity and less readily sensitized
Htr1btm1Rhn/Htr1btm1Rhn
        involves: 129S2/SvPas * 129S6/SvEvTac
  • behavior/neurological phenotype
    • abnormal operant conditional behavior (MGI Ref ID J:96223)
      • "acquired autoshaping" occurs more rapidly


Strain of Origin: 129S6/SvEvTac

Strain genetic background: 129S6/SvEvTac

Strain Development: Donor keep this strain for 15 years as a 129 SvEvTac background.

Suggested Control Mice:

  • Wildtype littermates

  • Developmental Biology
  • Neurobiology

Donor: Rene Hen, Ph.D., Columbia University

Primary Reference:

  • Saudou F, Amara DA, Dierich A, LeMeur M, Ramboz S, Segu L, Buhot MC, Hen R. Enhanced aggressive behavior in mice lacking 5-HT1B receptor. Science. 1994 Sep 23;265(5180):1875-8. (Medline PMID: 8091214)
  • Rocha BA, Scearce-Levie K, Lucas JJ, Hiroi N, Castanon N, Crabbe JC, Nestler EJ, Hen R. Increased vulnerability to cocaine in mice lacking the serotonin-1B receptor. Nature. 1998 May 14;393(6681):175-8. (Medline PMID: 9603521)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
030040-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030040-UNC-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.