Strain Name:
B6N;B6J-Creb1tm1Nes/Mmmh
Stock Number:
030042-MU
Citation ID:
RRID:MMRRC_030042-MU
Other Names:
Previously: C57BL/6-Creb1tm1Nes/Mmmh

Strain Information

Creb1tm1Nes
Name: cAMP responsive element binding protein 1; targeted mutation 1, Eric Nestler
Synonyms: Crebneo-loxP, Floxed CREB
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Conditional
Creb1
Name: cAMP responsive element binding protein 1
Synonyms: 3526402H21Rik, Creb, 2310001E10Rik, Creb-1
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Conditional
NCBI: 12912
HGNC: HGNC:2345
Homologene: 3223
Genetic Alterations
A loxP site and FRT flanked neo cassette were inserted upstream of exon 2 and a loxP site was inserted downstream of exon 2 via homologous recombination.
Genotype Determination
ES Cell Line
81E10-40F - Cell & Molecular Technologies
Phenotype
Homozygous: Homozygote for floxed Creb1 gene. Once crossed to Cre-expressing line, the Creb1 gene is knocked out.
Heterozygous: Not characterized.
Mammalian Phenotype Terms
Allelic Composition: Creb1tm1Nes/Creb1tm1Nes (Genetic Background: involves: C57BL/6J )

Strain Development
C57BL/6J-derived ES cells (81E10-40F - Cell & Molecular Technologies). Chimeras were mated to C57BL/6N from Charles River. PCR-screened heterozygotes were mated to produce homozygotes, which were sib-mated until donation to the MMRRC.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Research Tools
  • Drug Abuse
  • Depression
Donor
Eric J. Nestler, M.D., Icahn School of Medicine at Mount Sinai.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross with C57BL/6J
Breeding Scheme(s)
Random homozygote to homozygote
Generation
"N1" (C57BL/6N), F?
Overall Breeding Performance
Good; breed and act like C57BL/6
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 8 months
Bred to Homozygosity
Yes
Average litter size
6 pups
Recommended wean age
3 weeks
Average Pups Weaned

6 pubs

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030042-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
030042-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.