Strain Name:
STOCK Nkx2-1tm1Shk/Mmnc
Stock Number:
030044-UNC
Citation ID:
RRID:MMRRC_030044-UNC

Strain Information

Nkx2-1tm1Shk
Name: NK2 homeobox 1; targeted mutation 1, Shioko Kimura
Synonyms: Nkx2.1-, Titf1-, T/ebp null, T/EBP/NKX2.1-null
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Knockout
Nkx2-1
Name: NK2 homeobox 1
Synonyms: T/EBP, Ttf-1, thyroid transcription factor-1, tinman, thyroid-specific enhancer-binding protein, Titf1
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knockout
NCBI: 21869
Homologene: 2488
Genetic Alterations
A neo cassette was inserted into the middle of the homeodomain sequence.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    J1 derived from 129S4/SvJae
    Phenotype
    Homozygous: Die at birth mainly due to severely hypoplastic lungs, which do not allow pups to breath. In addition, they have severely deformed hypothalamus, and missing the pituitary and the thyroid.

    Heterozygous: No obvious abnormalities in mice unless challenged or carefully examined. They have a slightly higher level of TSH (hypothyroidism) and neurological disorder, which can be revealed when they are subjected to moter coordination test such as rotarod.
    Mammalian Phenotype Terms
    Allelic Composition: Nkx2-1tm1Shk/Nkx2-1tm1Shk (Genetic Background: B6.129S4-Nkx2-1tm1Shk )

    Allelic Composition: Nkx2-1tm1Shk/Nkx2-1tm1Shk (Genetic Background: involves: 129S4/SvJae )

    Allelic Composition: Nkx2-1tm1Shk/Nkx2-1tm1Shk (Genetic Background: involves: 129S4/SvJae * Black Swiss )

    Allelic Composition: Nkx2-1tm1Shk/Nkx2-1+ (Genetic Background: involves: 129S4/SvJae )

    Allelic Composition: Nkx2-1tm1Shk/Nkx2-1+ (Genetic Background: involves: 129S4/SvJae * C57BL/6 * CD-1 )

    MeSH Terms
    • Adrenal Cortex/anatomy & histology
    • Adrenal Cortex/embryology
    • Adrenal Cortex/pathology
    • Animals
    • Animals, Newborn
    • Cells, Cultured
    • Embryo, Mammalian/cytology
    • Embryonic Induction/genetics
    • Gene Expression Regulation, Developmental
    • Genes, Lethal
    • Genetic Vectors
    • Homozygote
    • In Situ Hybridization
    • Lung/anatomy & histology
    • Lung/embryology
    • Lung/pathology
    • Mice
    • Mice, Inbred C57BL
    • Mice, Transgenic
    • Nuclear Proteins/genetics
    • Pituitary Gland/anatomy & histology
    • Pituitary Gland/embryology
    • Pituitary Gland/pathology
    • Prosencephalon/anatomy & histology
    • Prosencephalon/embryology
    • Prosencephalon/pathology
    • Thyroid Gland/anatomy & histology
    • Thyroid Gland/embryology
    • Thyroid Nuclear Factor 1
    • Transcription Factors/genetics
    Strain Development
    129S4/SvJae founder male was bred to C57BL/6N females. After pups were sib- or randomly intra-strain mated for several generations, they were then backcrossed to Black Swiss from Taconic. After more than 10 generations backcrossed, they became very poor breeders. They were again crossed with earlier backcrossing generation of wild-type siblings. Donor does not have clear idea how many generations these wild-type mice were backcrossed to Black Swiss when they were used for mating with N10(11/12) Black Swiss T/ebp- heterozygous mice. The mixed line mice were under randomly intra-strain cross.
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Developmental Biology
    • Endocrine Deficiency
    • Models for Human Disease
    • Neurobiology
    • Pulmonary
    Donor
    Shioko Kimura, Ph.D., National Institutes of Health, National Cancer Institute
    Primary Reference
    Kimura S, Hara Y, Pineau T, Fernandez-Salguero P, Fox CH, Ward JM, Gonzalez FJ. The T/ebp null mouse: thyroid-specific enhancer-binding protein is essential for the organogenesis of the thyroid, lung, ventral forebrain, and pituitary. Genes Dev. 1996 Jan 1;10(1):60-9. (Medline PMID: 8557195)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    030044-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.