Strain Name:
STOCK Lhx4tm1Ssp/Mmmh
Stock Number:
030090-MU
Citation ID:
RRID:MMRRC_030090-MU

Strain Information

Lhx4tm1Ssp
Name: LIM homeobox protein 4; targeted mutation 1, S Steven Potter
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Lhx4
Name: LIM homeobox protein 4
Synonyms: Gsh-4, Gsh4, A330062J17Rik
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knockout
NCBI: 16872
Homologene: 56497
Genetic Alterations
Homologous recombination between pMCKOGsh-4 and the Lhx4target generated a 0.7 kb deletion at the Lhx4 locus, which included sequences encoding the Lhx4 homeobox.
Genotype Determination
ES Cell Line
D3 derived from 129S2/SvPas
Phenotype

Homozygous phenotype: Homozygous lethal

Heterozygous phenotype: Undetermined

Mammalian Phenotype Terms
Allelic Composition: Lhx4tm1Ssp/Lhx4tm1Ssp (Genetic Background: involves: 129S2/SvPas * CF1 )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Central Nervous System/growth & development
  • Central Nervous System/physiology
  • DNA Mutational Analysis
  • DNA-Binding Proteins/genetics
  • Genes, Homeobox/genetics
  • Genes, Lethal/genetics
  • Homeodomain Proteins
  • In Situ Hybridization
  • LIM-Homeodomain Proteins
  • Mice
  • Molecular Sequence Data
  • Phenotype
  • Protein Binding
  • Sequence Homology, Amino Acid
  • Structure-Activity Relationship
  • Tissue Distribution
  • Transcription Factors/genetics
  • Embryo, Mammalian
  • Forkhead Box Protein L2
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/metabolism
  • Forkhead Transcription Factors/physiology
  • Gene Expression Regulation, Developmental
  • Glycoprotein Hormones, alpha Subunit/metabolism
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Models, Biological
  • Pituitary Gland/embryology
  • Pituitary Gland/metabolism
  • Pituitary Gland, Anterior/embryology
  • Pituitary Gland, Anterior/growth & development
  • Pituitary Gland, Anterior/metabolism
  • Transcription Factors/physiology
  • Apoptosis/physiology
  • Body Patterning/physiology
  • Cell Survival
  • Dwarfism/genetics
  • Embryonic and Fetal Development/physiology
  • Mice, Knockout
  • Mice, Mutant Strains
  • Nerve Tissue Proteins/genetics
  • Cell Differentiation
  • Cell Division
  • Cell Lineage
  • Embryonic and Fetal Development/genetics
  • Genes, Homeobox
  • Mutation
  • Pituitary Gland/chemistry
  • Pituitary Gland/cytology
  • Pituitary Hormones/analysis
  • Pituitary Hormones/genetics
  • Stem Cells/cytology
  • Transcription Factors
  • Genes
  • Growth Hormone-Releasing Hormone/genetics
  • Hematopoiesis
  • Pituitary Hormones/metabolism
  • RNA, Messenger/genetics
  • Saccharomyces cerevisiae
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Steven S. Potter, Ph.D., Cincinnati Children's Hospital Medical Center
Sally A. Camper, Ph.D., University of Michigan
Primary Reference
  • Li H, Witte DP, Branford WW, Aronow BJ, Weinstein M, Kaur S, Wert S, Singh G, Schreiner CM, Whitsett JA, et al. Gsh-4 encodes a LIM-type homeodomain, is expressed in the developing central nervous system and is required for early postnatal survival. EMBO J. 1994 Jun 15. (Medline PMID: 7913017)
  • Ellsworth BS; Egashira N; Haller JL; Butts DL; Cocquet J; Clay CM; Osamura RY; Camper SA, FOXL2 in the pituitary: molecular, genetic, and developmental analysis., Mol Endocrinol 2006 Nov;20(11):2796-805 (Medline PMID: 16840539)
  • Raetzman LT; Ward R; Camper SA, Lhx4 and Prop1 are required for cell survival and expansion of the pituitary primordia., Development 2002 Sep;129(18):4229-39 (Medline PMID: 12183375)
  • Sheng HZ; Moriyama K; Yamashita T; Li H; Potter SS; Mahon KA; Westphal H, Multistep control of pituitary organogenesis., Science 1997 Dec 5;278(5344):1809-12 (Medline PMID: 9388186)
  • Li H; Zeitler PS; Valerius MT; Small K; Potter SS, Gsh-1, an orphan Hox gene, is required for normal pituitary development., EMBO J 1996 Feb 15;15(4):714-24 (Medline PMID: 8631293)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030090-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
030090-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.