Strain Name:
C.Cg-Tg(TG-TSHR)50.6Smcl/Mmucd
Stock Number:
030109-UCD
Citation ID:
RRID:MMRRC_030109-UCD
Other Names:
A-subunit transgenic (line 50.6), C.Cg-Tg(TG-TSHR)50.6Smcl/Mmcd

Strain Information

Tg
Name: thyroglobulin
Synonyms: Tgn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 21819
Homologene: 2430
Tshr
Name: thyroid stimulating hormone receptor
Synonyms: hyt, hypothroid, pet
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22095
Homologene: 315
Tg(TG-TSHR)50.6Smcl
Name: transgene insertion 50.6, Sandra M McLachlan
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The human TSHR A- subunit cDNA (amino acid residues 1-289)(13) with a 6 histidine tag at the C-terminus was excised from pSV2-ECE-TSHR-289-6H-dhfr with EcoR I and Xba I and subcloned into the same sites in pcDNA3. This intermediate plasmid (pcDNA3-289), containing a Kpn I site 5' to the insert, was mutagenized to introduce a Kpn I site 3' to the bovine growth hormone polyA tail. The bovine thyroglobulin (Tg) promoter in the plasmid TgSK+ (amino acids -2036 to +9; Gene Bank accession No. M35823) was restricted with BamH I; the released 2 kb fragment was inserted in the correct orientation into pcDNA3-289 to generate pTg-TSHR-289. After excision with Kpn I, purified pTg-TSHR-289 was micro-injected (UC-Irvine Transgenic Mouse Facility, Irvine, CA) into oocytes from CB6F1 x CB6F1 mice (F1 of a BALB/c female and a C57BL/6 male; Harlan, Indianapolis, IN).
Phenotype
Homozygous: Not determined

Hemizygous: Normal thyroid architectures and serum thyroxine (T4). Unlike wild-type littermates, or related transgenics that express low concentrations of A- subunit protein (MMRRC:014125), 50.6 line mice have the highest human A-subunit protein content and they are are unresponsive (tolerant) to immunization with high dose A-subunit adenovirus.
MeSH Terms
  • Adenoviridae/genetics
  • Animals
  • Autoantibodies/immunology
  • Graves Disease/immunology
  • Graves Disease/pathology
  • Hashimoto Disease/immunology
  • Hashimoto Disease/pathology
  • Humans
  • Hyperthyroidism/immunology
  • Hyperthyroidism/pathology
  • Immunization
  • Immunoglobulins, Thyroid-Stimulating/metabolism
  • Immunohistochemistry
  • Mice
  • Mice, Transgenic
  • Receptors, Thyrotropin/genetics
  • Receptors, Thyrotropin/immunology
  • T-Lymphocytes, Regulatory/immunology
  • T-Lymphocytes, Regulatory/metabolism
  • Thyroid Gland/immunology
  • Thyroid Gland/metabolism
  • Adjuvants, Immunologic/metabolism
  • Amino Acid Sequence
  • Antibodies/immunology
  • Epitopes/immunology
  • Immune Tolerance/immunology
  • Immunoglobulin G/immunology
  • Iodide Peroxidase/metabolism
  • Molecular Sequence Data
  • Peptides/chemistry
  • Peptides/immunology
  • Protein Subunits/chemistry
  • Protein Subunits/immunology
  • Receptors, Thyrotropin/chemistry
  • Thyroiditis/blood
  • Thyroiditis/immunology
  • Thyroxine/blood
Strain Development
Founder transgenic crossed to BALB/cJ. Offspring of each generation, crossed to BALB/cJ
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunology and Inflammation
  • Models for Human Disease
    • Graves' disease
Donor
Sandra M. McLachlan, Ph.D., Cedars-Sinai Medical Center
Primary Reference
  • McLachlan SM; Nagayama Y; Pichurin PN; Mizutori Y; Chen CR; Misharin A; Aliesky HA; Rapoport B, The link between Graves' disease and Hashimoto's thyroiditis: a role for regulatory T cells., Endocrinology 2007 Dec;148(12):5724-33. (Medline PMID: 17823263).
  • McLachlan SM, Aliesky HA, Chen CR, Chong G, Rapoport B. Breaking tolerance in transgenic mice expressing the human TSH receptor A-subunit: thyroiditis, epitope spreading and adjuvant as a 'double edged sword'. PLoS One. 2012;7(9):e43517. Epub 2012 Sep 7. (Medline PMID: 22970131).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
White
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • BALB/cJ female x Hemizygous male or reciprocal mating
Generation
N10+
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Unknown Unknown
Homozygotes are fertile: Unknown Unknown
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: No data No data
Average litter size
7
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030109-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030109-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
030109-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.