Strain Name:
B6;129P2-Npnttm1Lfr/Mmmh
Stock Number:
030111-MU
Citation ID:
RRID:MMRRC_030111-MU
Other Names:
nephronectin

Strain Information

Npnttm1Lfr
Name: nephronectin; targeted mutation 1, Louis F Reichardt
Synonyms: Npntfloxneo
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Conditional
Npnt
Name: nephronectin
Synonyms: 1110009H02Rik, POEM
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Conditional
NCBI: 114249
Homologene: 14138
Genetic Alterations
Exon 1 was flanked by loxP sites. A neo and a third loxP site were inserted downstream of the floxed exon.
Genotype Determination
ES Cell Line
E14 derived from 129P2/OlaHsd
Phenotype
Homozygous: mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in the kidney results in kidney agenesis or hypoplasia. Mice homozygous for the targeted mutation are viable and fertile.

Heterozygotes: viable and fertile.
Mammalian Phenotype Terms
Allelic Composition: Npnttm1Lfr/Npnttm1Lfr (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

MeSH Terms
  • Adaptor Proteins, Signal Transducing
  • Alleles
  • Animals
  • Animals, Newborn
  • Basement Membrane/embryology
  • Basement Membrane/metabolism
  • Extracellular Matrix Proteins/deficiency
  • Extracellular Matrix Proteins/genetics
  • Extracellular Matrix Proteins/metabolism
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Regulatory Networks
  • Glial Cell Line-Derived Neurotrophic Factor/genetics
  • Glial Cell Line-Derived Neurotrophic Factor/metabolism
  • Integrins/deficiency
  • Integrins/genetics
  • Integrins/metabolism
  • Kidney/blood supply
  • Kidney/embryology
  • Kidney/metabolism
  • Membrane Proteins/deficiency
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Mice
  • Mice, Knockout
  • Phosphoproteins/deficiency
  • Phosphoproteins/genetics
  • Phosphoproteins/metabolism
Strain Development
The floxneo targeting construct was electroporated into E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts producing chimera and subsequent germline transmission.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Research Tool
Donor
Louis F. Reichardt, Ph.D., University of California, San Francisco
Primary Reference
Linton JM, Martin GR, Reichardt LF. The ECM protein nephronectin promotes kidney development via integrin alpha8beta1-mediated stimulation of Gdnf expression. Development. 2007 Jul;134(13):2501-9. Epub 2007 May 30. (Medline PMID: 17537792)

Colony and Husbandry Information

The MMRRC strain 030248-MU, B6;129P2-Npnttm1.1Lfr/Mmmh has the floxed fragment removed and is null for Npnt.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030111-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
030111-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.