Strain Name    : C57BL/6J-Card11m1Btlr/Mmucd

Stock Number : 030114-UCD

Other Names   : king, C57BL/6J-Card11m1Btlr/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Card11m1Btlr
Name: mutation 1, Bruce Beutler
Chromosome: 5
Alteration at locus: Chemically Induced
Gene Symbol: Card11
Name: caspase recruitment domain family, member 11
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations:
ENU-induced A to T transversion at position 1791 of the Carma1/Card11 gene, in exon 12.

Genotype Determination:

ES Cell Line:

Additional mutations found on this strain:

AlleleSourceChrCoordTypePredicted EffectPPH Score
unmodulated APN 5 140897997 missense probably damaging 1.00
IGL00961:Card11 APN 5 140899709 missense possibly damaging 0.60
IGL01731:Card11 APN 5 140882302 missense possibly damaging 0.64
IGL01782:Card11 APN 5 140927726 missense probably benign 0.01
IGL01935:Card11 APN 5 140883546 missense probably damaging 1.00
IGL01991:Card11 APN 5 140913378 missense possibly damaging 0.84
IGL02447:Card11 APN 5 140906924 missense possibly damaging 0.81
IGL02583:Card11 APN 5 140878126 missense probably benign 0.01
hubei UTSW 5 140906767 missense probably damaging 0.98
May UTSW 5 140876495 nonsense probably null
R0046:Card11 UTSW 5 140908524 missense possibly damaging 0.92
R0285:Card11 UTSW 5 140887101 missense probably damaging 1.00
R0452:Card11 UTSW 5 140880370 missense probably benign 0.01
R1486:Card11 UTSW 5 140876519 missense probably benign 0.00
R1710:Card11 UTSW 5 140902905 nonsense probably null
R1733:Card11 UTSW 5 140906633 missense possibly damaging 0.88
R1817:Card11 UTSW 5 140885560 missense probably benign 0.00
R1818:Card11 UTSW 5 140885560 missense probably benign 0.00
R2436:Card11 UTSW 5 140882362 missense possibly damaging 0.89
R2904:Card11 UTSW 5 140889133 missense possibly damaging 0.50
R3706:Card11 UTSW 5 140887135 missense probably damaging 0.99
R3708:Card11 UTSW 5 140887135 missense probably damaging 0.99
V7732:Card11 UTSW 5 140876495 nonsense probably null
X0067:Card11 UTSW 5 140885592 missense possibly damaging 0.60

Phenotype

Homozygous phenotype: -No CD8+ T cell response after immunization and subsequent challenge with antigen- specific targets. - No interferon-gamma production by CD8+ T cells after immunization and stimulation with antigen in vitro. - No proliferation or CD25 upregulation after T cell receptor stimulation of T cells. - No CD4+Foxp3+ regulatory T cells in thymus, and greatly reduced numbers in spleen. - Reduced basal serum immunoglobulin levels. - Impaired B cell proliferation. - No antigen-specific IgG responses after immunization with ovalbumin. - Reduced numbers and percentages of natural killer (NK), NKT, gamma/delta T, memory CD4+ T, and mature B cells.

Heterozygous phenotype:

Mammalian Phenotype Terms:
Allelic Composition: Card11m1Btlr/Card11m1Btlr (Genetic Background: C57BL/6J-Card11m1Btlr )

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: The founder homozygous ENU-induced mutant was backcrossed to C57BL/6J. Heterozygote progeny were mated to the original mutant and homozygote progeny from this cross have subsequently been intercrossed to maintain the stock.

Suggested Control Mice: Wild-type littermates

  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Immunology and Inflammation
  • Virology

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

  • Barnes MJ; Krebs P; Harris N; Eidenschenk C; Gonzalez-Quintial R; Arnold CN; Crozat K; Sovath S; Moresco EM; Theofilopoulos AN; Beutler B; Hoebe K, Commitment to the regulatory T cell lineage requires CARMA1 in the thymus but not in the periphery., PLoS Biol 2009 Mar 3;7(3):e51 (Medline PMID: 19260764)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Donor notes homozygotes are susceptible to infection and dermatitis.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
030114-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030114-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030114-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.