Strain Name:
C57BL/6J-Alx4m1Btlr/Mmucd
Stock Number:
030202-UCD
Citation ID:
RRID:MMRRC_030202-UCD
Other Names:
goofy, C57BL/6J-Alx4m1Btlr/Mmcd
Major Collection:

Gene Information

Alx4m1Btlr
Name: aristaless-like homeobox 4; mutation 1, Bruce Beutler
Synonyms: goofy
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
Alx4
Name: aristaless-like homeobox 4
Synonyms: Aristaless-like 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 11695
HGNC: HGNC:450
Homologene: 7229
Genetic Alterations
The goofy allele contains an ENU-induced A to G transition at position 1010 of the Alx4 gene (Genbank Accession NM_007442), and results in a histidine 272 to arginine substitution in the encoded protein.
Genotype Determination
Phenotype
Homozygous: Homozygous goofy mice are viable and exhibit delayed or absent fur growth on the back and a snout that is shorter and slightly curved (upturned). Goofy mice display preaxial polydactyly (formation of one or more extra anterior digits) in the hind limbs, with an extra toe that is either a duplication of digit I (big toe) or a mirror image of digit II. The forelimbs appear normal. Homozygous animals are fertile.

Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Alx4m1Btlr/Alx4m1Btlr (Genetic Background: C57BL/6J-Alx4m1Btlr )

Strain Development
Mutant backcrossed to C57BL/6J several generations; all subsequent crosses only on C57BL/6J background.
Suggested Control Mice
Wild-type littermates
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Donor
Bruce Beutler, M.D., The Scripps Research Institute
Primary Reference
Moresco EM, Rutschmann S, Du X, Beutler B. Record for goofy, updated 10 Oct 2007. MUTAGENETIX (TM), B. Beutler and colleagues, Department of Genetics, The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Donor notes Homozygote females are fertile, but make poor mothers. Therefore, homozygous males are normally bred with heterozygous females.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030202-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030202-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
030202-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.