Strain Detail Sheet

Strain Name:
B6;129X1-Dctn1tm1.1Cai/Mmmh
Stock Number:
030222-MU
Citation ID:
RRID:MMRRC_030222-MU
Other Names:
dynactin 1

Strain Information

Gene Details

Dctn1tm1.1Cai
Name: dynactin 1; targeted mutation 1.1, Huaibin Cai
Synonyms: Dctn1delta
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
Dctn1
Name: dynactin 1
Synonyms: p150, Glued, p150glued
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knock-In
NCBI: 13191
HGNC: HGNC:2711
Homologene: 3011
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Genetic Alterations
To construct the knock-in targeting vector, a 3.1 kb HindIII/SpeI fragment containing exons 2 and 3 of Dctn1 was modified by introduction of a new DdeI site in exon 2 by replacement of nucleotides GGGC with CTCA using QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA), resulting in the substitution of encoded amino acid Glycine with Serine (Dctn1/m). To construct the conditional knockout construct, one copy of Loxp site was inserted into the HindIII site in front of exon 2 and a 3.4 kb XbaI fragment, containing the neomycin-resistance gene flanked with two Loxp sites (lnl), was inserted into the SpeI site immediately following exon 3 (Dctn1/lnl).
Genotype Determination
ES Cell Line
Not specified

Strain Description

Phenotype
Homozygous: No Dctn1m/m offspring were obtained from Dctn1+/m crosses. The ratio of wild-type and Dctn1+/m mice was 1:2, suggesting that the homozygous Dctn1m/m mice are embryonically lethal. Donor then set up timed pregnant mating of heterozygous Dctn1+/m mice and collected embryos at different gestation stages. The Dctn1m/m embryo had severe gastrulation problems. The development of Dctn1m/m embryos was likely arrested around 8.0 dpc in which the neural tube was only partially formed and no typical somite could be identified.

Heterozygous: Heterozygous knock-in mice, which developed normally, displayed motor neuron disease-like phenotypes after 10 months of age, including excessive accumulation of cytoskeletal and synaptic vesicle proteins at neuromuscular junctions, loss of spinal motor neurons, increase of reactive astrogliosis, and shortening of gait compared with wild-type littermates and age-matched p150glued heterozygous knockout mice.
Mammalian Phenotype Terms
Allelic Composition: Dctn1tm1.1Cai/Dctn1tm1.1Cai (Genetic Background: involves: 129X1/SvJ * C57BL/6 * FVB/N )
MeSH Terms
  • Animals
  • Dynactin Complex
  • Female
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Microtubule-Associated Proteins/genetics
  • Microtubule-Associated Proteins/physiology
  • Motor Neuron Disease/genetics
  • Motor Neuron Disease/metabolism
  • Mutation, Missense
  • Pregnancy
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Apoptosis
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor
Huaibin Cai, Ph.D., National Institutes of Health, National Institute on Aging
Primary Reference
Lai C, Lin X, Chandran J, Shim H, Yang WJ, Cai H. The G59S mutation in p150(glued) causes dysfunction of dynactin in mice. J Neurosci. 2007 Dec 19;27(51):13982-90. (Medline PMID: 18094236)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
030222-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
030222-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.