Name: targeted mutation 3, Henry Lester
Alteration at locus: Knock-In
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Alteration at locus: Knock-In
ES Cell Line: CJ7 derived from B6;129S1
Homozygous phenotype: Alpha 4
Nicotinic receptors are flourescent due to the presence of fused YFP.
The mice behave normally in every assay tested as compared to WT.
Heterozygous phenotype: Alpha4 Nicotinic receptor are flourescent due to the presence of fused YFP. Flourescence level is half that of Homozygotes.
Strain of Origin: Sv129
Strain genetic background: C57BL/6
Strain Development: All neo-deleted clones were sequenced to confirm the presence of the YFP mutation and deletion of the neo cassette. Karyotype analysis was used to verify normal chromosome number. Two neo-deleted Alpha 4YFP clones were injected into C57BL/6 blastocysts, and chimeras were established. Chimeras were mated with wild-type animals and germline transmission was established as assessed by PCR analysis of tail DNA and sequence analysis. The F1 generation was then baccrossed to C57 BL/6 to achieve N10 generation which was congenic.
Suggested Control Mice:
Donor: Henry A. Lester, Ph.D., California Institute of Technology
Nashmi R, Xiao C, Deshpande P, McKinney S, Grady SR, Whiteaker P, Huang Q, McClure-Begley T, Lindstrom JM, Labarca C, Collins AC, Marks MJ, Lester HA. Chronic nicotine cell specifically upregulates functional alpha 4* nicotinic receptors: basis for both tolerance in midbrain and enhanced long-term potentiation in perforant path. J Neurosci. 2007 Aug 1;27(31):8202-18. (Medline PMID: 17670967)
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.