Strain Detail Sheet

Strain Name    :

STOCK-Tbr1tm1Jlr/Mmucd

Stock Number :

030263-UCD

Other Names   :

Tbr1(+/-), STOCK-Tbr1tm1Jlr/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Tbr1tm1Jlr
Name: targeted mutation 1, John L Rubenstein
Alteration at locus: Knockout
Gene Symbol: Tbr1
Name: T-box brain gene 1
Chromosome: 2
Alteration at locus: Knockout

Genetic Alterations:
Deletion of exons 2 and 3 Insertion of PGK neo

Genotype Determination:

ES Cell Line: JM-1 derived from B6;129X1

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Lethal P0/P1, Cerebral Cortex abnormalities

Heterozygous phenotype: Unknown


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Tbr1tm1Jlr/Tbr1tm1Jlr
        involves: 129X1/SvJ
  • mortality/aging
    • complete postnatal lethality (MGI Ref ID J:51888)
      • animals die by the second postnatal day
  • behavior/neurological phenotype
    • abnormal eating behavior (MGI Ref ID J:51888)
      • homozygous pups do not feed
  • nervous system phenotype
    • abnormal forebrain morphology (MGI Ref ID J:51888)
      • disruption of the laminar structure of the cortical neurons, reduced number of preplate neurons and their derivatives
      • abnormal olfactory bulb development (MGI Ref ID J:51888)
        • failure to form normal projection neurons (mitral and tufted cells) in the olfactory bulb
      • abnormal olfactory bulb external plexiform layer morphology (MGI Ref ID J:51888)
        • the granule cell layer and glomerular/external plexiform layer appear as a single layer
        • abnormal olfactory bulb tufted cell morphology (MGI Ref ID J:51888)
          • failure to form normal tufted cells
      • abnormal olfactory bulb glomerular layer morphology (MGI Ref ID J:51888)
        • the glomerular layer contains 3-4 smaller glomeruli in its radial dimension rather than the 1-2 glomeruli seen in wild-type mice
      • abnormal olfactory bulb interneuron morphology (MGI Ref ID J:51888)
        • disorganization of the interneuron cell bodies due to absence of an intact mitral cell layer
        • the granule cell layer and glomerular/external plexiform layer appear as a single layer
      • abnormal olfactory bulb mitral cell layer morphology (MGI Ref ID J:51888)
        • absence of an intact mitral cell layer
        • abnormal mitral cell morphology (MGI Ref ID J:51888)
          • failure to form normal mitral cells
      • abnormal olfactory cortex morphology (MGI Ref ID J:51888)
        • hypoplastic olfactory cortex
        • abnormal olfactory tract morphology (MGI Ref ID J:51888)
          • absence of axonal projections from the olfactory bulb to the primary olfactory cortex consistent with absence of projection neurons
      • olfactory bulb hypoplasia (MGI Ref ID J:51888)
        • due to reduced number of cells
    • abnormal sensory neuron projections (MGI Ref ID J:51888)
      • striking reduction in mature olfactory bulb projection neurons
    • decreased brain size (MGI Ref ID J:51888)

Strain of Origin: C57BL/6J

Strain genetic background: Crl:CD1(ICR) mixed with C57BL/6J

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Neurobiology

Strain Origin

Donor: John Rubenstein, M.D., Ph.D., University of California at San Francisco

Primary Reference:

  • Hevner RF; Shi L; Justice N; Hsueh Y; Sheng M; Smiga S; Bulfone A; Goffinet AM; Campagnoni AT; Rubenstein JL, Tbr1 regulates differentiation of the preplate and layer 6., Neuron 2001 Feb;29(2):353-66 (Medline PMID: 11239428)
  • Bulfone A; Wang F; Hevner R; Anderson S; Cutforth T; Chen S; Meneses J; Pedersen R; Axel R; Rubenstein JL, An olfactory sensory map develops in the absence of normal projection neurons or GABAergic interneurons., Neuron 1998 Dec;21(6):1273-82 (Medline PMID: 9883721)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030263-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030263-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030263-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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