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Strain Name:
B6;129-Gata2tm1Sac/Mmmh
Stock Number:
030290-MU
Citation ID:
RRID:MMRRC_030290-MU

Strain Information

Gata2tm1Sac
Name: GATA binding protein 2; targeted mutation 1, Sally A Camper
Synonyms: Gata2f
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Gata2
Name: GATA binding protein 2
Synonyms: Gata-2
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knockout
NCBI: 14461
HGNC: HGNC:4171
Homologene: 32030
Genetic Alterations
Neo cassette flanked by FRT sites was cloned upstream of exon five which encodes the C- terminal zinc finger domain.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Homozygous floxed= no abnormalities systemic null= embryonic lethal, similar to described phenotype in Tsai et al., Nature 371:221, 1994.

Heterozygous: Same as wild-type
MeSH Terms
  • Animals
  • Animals, Newborn
  • Base Sequence
  • Body Weight
  • DNA/genetics
  • Female
  • Follicle Stimulating Hormone/blood
  • GATA2 Transcription Factor/deficiency
  • GATA2 Transcription Factor/genetics
  • GATA2 Transcription Factor/physiology
  • GATA3 Transcription Factor/genetics
  • Gonadotropins, Pituitary/biosynthesis
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Orchiectomy
  • Pituitary Gland/pathology
  • Pituitary Gland/physiopathology
  • Pregnancy
  • Thyroidectomy
  • Thyrotropin/biosynthesis
  • Transcription, Genetic
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
  • Cardiovascular
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Hematology
  • Internal/Organ
  • Models for Human Disease
Donor
Sally A. Camper, Ph.D., University of Michigan
Primary Reference
Charles MA, Saunders TL, Wood WM, Owens K, Parlow AF, Camper SA, Ridgway EC, Gordon DF. Pituitary-specific Gata2 knockout: effects on gonadotrope and thyrotrope function. Mol Endocrinol. 2006 Jun;20(6):1366-77. Epub 2006 Mar 16. (Medline PMID: 16543408)
Strain Development
Germline transmitters were mated to flpE (Rodriguez et al., Nat Gen 25:139, 2000) to remove the neo cassettte.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu

Order Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@missouri.edu for more details.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030290-MU-HET-F
030290-MU-HET-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
$290.00 / Non-Profit Per Mouse The mmrrc@missouri.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
030290-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.