Strain Name:
C57BL/6-Tg(CAG-mRFP,-CXCR1,-luc/EGFP)1Mngn/Mmucd
Stock Number:
030294-UCD
Citation ID:
RRID:MMRRC_030294-UCD
Other Names:
RCLG3A

Strain Information

mRFP
Name: monomeric red fluorescent protein
Type: DNA Segment
Species: Discosoma sp. (mushroom coral)
Chromosome:
luc/EGFP
Name: Renilla luciferase-GFP fusion
Type: Gene
Species: Multi-species
Chromosome:
Tg(CAG-mRFP,-CXCR1,-luc/EGFP)1Mngn
Name: transgene insertion 1, Manuela Martins-Green
Synonyms: RCLG3A, Tg(ACTB-mRFP,-IL8RA,-luc/EGFP)1Mngn
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
A transgene carrying following cassetts with specified order: CMV promoter-loxP- monomeric Red Fluorescent Protein ORF-3x polyA signal-loxP-hCXCR1 ORF - IRES -eGFP/Luciferase fusion ORF-polyA signal.
Genotype Determination
Phenotype
Homozygous: The homozygous have ubiquitous expression of red fluorescent protein in all tissues.

Hemizygous: The hemizygous have ubiquitous expression of red fluorescent protein in all tissues.
MeSH Terms
  • Animals
  • Animals, Newborn/physiology
  • Estrogen Antagonists/pharmacology
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Integrases/genetics
  • Integrases/metabolism
  • Luciferases/metabolism
  • Luminescence
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • NIH 3T3 Cells
  • Phenotype
  • Plasmids
  • Proteins/physiology
  • RNA, Untranslated
  • Receptors, Estrogen/physiology
  • Tamoxifen/pharmacology
  • Tissue Distribution
  • Chemotaxis
  • Fibroblasts/cytology
  • Fibroblasts/metabolism
  • Genotype
  • Humans
  • Immunoblotting
  • Immunoprecipitation
  • Integrases
  • Interleukin-8/genetics
  • Interleukin-8/metabolism
  • Mice, Knockout
  • Receptors, Interleukin-8A/genetics
  • Receptors, Interleukin-8A/physiology
  • Skin/cytology
  • Skin/metabolism
Strain Development
Transgenic mice were generated on C57BL/6J genetic background. The positive hemizygous transgenic individuals were crossed with negative littermates to obtain hemizygous offspring.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Dermatology
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
Donor
Manuela Martins-Green, Ph.D., University of California, Riverside
Primary Reference

Zheng L, Njauw CN, Martins-Green M. A one-plasmid conditional color-switching transgenic system for multimodal bioimaging. Transgenic Res. 2008 Aug;17(4):741-7. Epub 2008 Jan 9. (Medline PMID: 18183494)

Zheng L, Njauw CN, Martins-Green M. A hCXCR1 transgenic mouse model containing a conditional color-switching system for imaging of hCXCL8/IL-8 functions in vivo. J Leukoc Biol. 2007 Nov;82(5):1247-56. Epub 2007 Aug 17. (Medline PMID: 17704296)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030294-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030294-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
030294-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.