Allele Symbol: Akap10Gt(RRC139)Byg
Name: gene trap RRC139, BayGenomics
Alteration at locus: Gene Trap
Gene Symbol: Akap10
Name: A kinase (PRKA) anchor protein 10
Alteration at locus: Gene Trap
ES Cell Line: E14Tg2a.4
Homozygous phenotype: In both
heterozygous and homozygous mutant mice derived from these mESCs, the
same Akap10 disruption increases the cardiac response to cholinergic
signals, suggesting a dominant interfering effect of the Akap10 mutant
allele. The mutant mice have cardiac arrhythmias and die prematurely.
Heterozygous phenotype: heterozygous phenotype is identical to homozygous for cholinergic response. however heterozygous mice have a less pronounced sudden death phenotype
Strain of Origin: 129
BayGenomics genetrap line
Strain genetic background: FVB/N
Strain Development: Originally was a BayGenomics genetrap cell line #RRC139, which would result in a 129 mix. However it has been backcrossed 9 generations to FVB/N
Suggested Control Mice:
Donor: Bruce Conklin, Ph.D., Gladstone Institute of Cardiovascular Disease
Tingley WG, Pawlikowska L, Zaroff JG, Kim T, Nguyen T, Young SG, Vranizan K, Kwok PY, Whooley MA, Conklin BR. Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation. Proc Natl Acad Sci U S A. 2007 May 15;104(20):8461-6. Epub 2007 May 7. (Medline PMID: 17485678)
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.