Strain Detail Sheet

Strain Name    :

FVB.129P2-Akap10Gt(RRC139)Byg/Mmmh

Stock Number :

030318-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Akap10Gt(RRC139)Byg
Name: gene trap RRC139, BayGenomics
Alteration at locus: Gene Trap
Gene Symbol: Akap10
Name: A kinase (PRKA) anchor protein 10
Chromosome: 11
Alteration at locus: Gene Trap

Genetic Alterations:
Genetrap mutation from BayGenomics (#RRC139) in that inserts BetaGeo cassette after Exon 12. This removes exons 13-15. This replaces the last 51 amino acids with BetaGeo residues.

Genotype Determination:

ES Cell Line: E14Tg2a.4

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: In both heterozygous and homozygous mutant mice derived from these mESCs, the same Akap10 disruption increases the cardiac response to cholinergic signals, suggesting a dominant interfering effect of the Akap10 mutant allele. The mutant mice have cardiac arrhythmias and die prematurely.

Heterozygous phenotype: heterozygous phenotype is identical to homozygous for cholinergic response. however heterozygous mice have a less pronounced sudden death phenotype


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Akap10Gt(RRC139)Byg/Akap10+
        involves: 129P2/OlaHsd
  • mortality/aging
    • premature death (MGI Ref ID J:121838)
      • on a 129P2/OlaHsd background, 25% of mice die within 1 year; mortality is observed only in adult animals
  • cardiovascular system phenotype
    • decreased heart rate (MGI Ref ID J:121838)
      • treatment of mice with cholinergic agonists results in greater slowing of heart rate compared to wild-type
Akap10Gt(RRC139)Byg/Akap10+
        involves: 129P2/OlaHsd * FVB/N
  • mortality/aging
    • mortality/aging (MGI Ref ID J:121838)
      • no early (<1 year) lethality is seen on this background
Akap10Gt(RRC139)Byg/Akap10Gt(RRC139)Byg
        involves: 129P2/OlaHsd
  • mortality/aging
    • premature death (MGI Ref ID J:121838)
      • on a 129P2/OlaHsd background, 50% of mice die within 1 year; mortality is observed only in adult animals
  • cardiovascular system phenotype
    • abnormal sinoatrial node conduction (MGI Ref ID J:121838)
      • mice show sinus pauses with junctional escape beats at a 40-fold inc reased frequency compared to wild-type (preceded by sinus slowing with prolonged P-P wave interval)
      • abnormal P wave (MGI Ref ID J:121838)
        • mutants exhibit 3 vs 0.2 in wild-type nonconductive P waves/hour (AV heartblock)
    • abnormal sinus arrhythmia (MGI Ref ID J:121838)
      • at greater than 10 weeks of age, unstressed mice show pronounced sinus arrhythmia
      • increased heart rate variability (MGI Ref ID J:121838)
        • unstressed mice older than 10 weeks have abnormally high beat-to-beat heart rate variability (HRV)
    • atrioventricular block (MGI Ref ID J:121838)
    • decreased heart rate (MGI Ref ID J:121838)
      • treatment of mice with cholinergic agonists results in greater slowing of heart rate compared to wild-type at more than 10 weeks of age
    • irregular heartbeat (MGI Ref ID J:121838)
  • nervous system phenotype
    • abnormal baroreceptor physiology (MGI Ref ID J:121838)
      • mice display stronger baroreflexes upon activation of vagus nerve with phenylephrine
Akap10Gt(RRC139)Byg/Akap10Gt(RRC139)Byg
        involves: 129P2/OlaHsd * FVB/N
  • mortality/aging
    • mortality/aging (MGI Ref ID J:121838)
      • no early (<1 year) lethality is seen on this background

Strain of Origin: 129
BayGenomics genetrap line

Strain genetic background: FVB/N

Strain Development: Originally was a BayGenomics genetrap cell line #RRC139, which would result in a 129 mix. However it has been backcrossed 9 generations to FVB/N

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Cardiovascular

Strain Origin

Donor: Bruce Conklin, Ph.D., Gladstone Institute of Cardiovascular Disease

Primary Reference:

Tingley WG, Pawlikowska L, Zaroff JG, Kim T, Nguyen T, Young SG, Vranizan K, Kwok PY, Whooley MA, Conklin BR. Gene-trapped mouse embryonic stem cell-derived cardiac myocytes and human genetics implicate AKAP10 in heart rhythm regulation. Proc Natl Acad Sci U S A. 2007 May 15;104(20):8461-6. Epub 2007 May 7. (Medline PMID: 17485678)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030318-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030318-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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