Allele Symbol: Cyp11b2tm2Hsk
Name: targeted mutation 2, Hyung-Suk Kim
Alteration at locus: Knockout
Gene Symbol: Cyp11b2
Name: cytochrome P450, family 11, subfamily b, polypeptide 2
Alteration at locus: Knockout
ES Cell Line: TC1/TC-1 derived from 129S6/SvEvTac
Homozygous: ~10mmHg higher blood pressure, higher plasma aldosterone, lower plasma potassium, greater renal expression of the the alpha subunit of epithelial sodium channel on high salt diet, increased cardiac hypertrophy and oxidative stress after angiotensin II infusion on high salt diet and resistance to the effects of low salt diet.
Heterozygous: not different from background strain
Strain of Origin: 129S6/SvEvTac
Strain genetic background: mixed 129S6/SvEvTac and C57BL6/J
Strain Development: Always maintained on SvEv strain, and also backcross 2 of C57BL/6J
Suggested Control Mice:
Donor: Oliver Smithies, Ph.D., Univerisity of North Carolina
Donor states: "Mice are very characteristic of the normal 129 strain in breeding efficiency and performance. They are more susceptible to hypertension with a moderately high salt diet."
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.