Strain Detail Sheet

Strain Name    :

C57BL/6J-Tlr9m3Btlr/Mmucd

Stock Number :

030343-UCD

Other Names   :

CpG3, C57BL/6J-Tlr9m3Btlr/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Tlr9m3Btlr
Name: mutation 3, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Tlr9
Name: toll-like receptor 9
Chromosome: 9
Alteration at locus: Chemically Induced

Genetic Alterations:
ENU-induced T to A transversion at position 747 of Tlr9 gene (Genbank Accession NM_031178); results in valine to glutamic acid change at position 214 of the TLR9 protein.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: The CpG3 phenotype was identified in a G3 screen for mutants with impaired response to Toll- like receptor (TLR) ligands. Peritoneal macrophages from homozygous CpG3 mice produce normal amounts of tumor necrosis factor (TNF)-alpha in response to all TLR ligands tested, except oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs). In response to CpG ODN treatment, homozygous CpG3 macrophages produce no TNF-alpha. In addition, na


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Tlr9m3Btlr/Tlr9m3Btlr
        C57BL/6J-Tlr9<m3Btlr>
  • immune system phenotype
    • abnormal macrophage physiology (MGI Ref ID J:128406)
      • peritoneal macrophages from homozygous mutant mice produce no tumor necrosis factor (TNF-) alpha in response to oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs)
      • TNF-alpha production by homozygous mutant macrophages is normal following stimulation with MALP-2 (macrophage-activating lipopeptide-2, a TLR2/6 ligand), Pam3CSK4 (a triacyl lipopeptide and TLR2/1 ligand), poly I:C (a TLR3 ligand), lipopolysaccharide (LPS, a TLR4 ligand), and R-848 (resiquimod, a single-stranded RNA mimetic and TLR7 ligand)
    • decreased B cell proliferation (MGI Ref ID J:128406)
      • naive B cells from peripheral blood of homozygous CpG3 mutant mice fail to proliferate upon stimulation with oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) in vitro, a response specific for CpG ODNs among other Toll-like receptor (TLR) agonists
  • hematopoietic system phenotype
    • decreased B cell proliferation (MGI Ref ID J:128406)
      • naive B cells from peripheral blood of homozygous CpG3 mutant mice fail to proliferate upon stimulation with oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) in vitro, a response specific for CpG ODNs among other Toll-like receptor (TLR) agonists

Strain of Origin: C57BL/6

Strain genetic background: C57BL/6

Strain Development: Original mutant is a C57BL/6J G3 ENU- induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cell Biology
  • Immunology and Inflammation
  • Virology

Strain Origin

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

  • Xiao N; Eidenschenk C; Krebs P; Brandl K; Blasius AL; Xia Y; Khovananth K; Smart NG; Beutler B, The Tpl2 mutation Sluggish impairs type I IFN production and increases susceptibility to group B streptococcal disease., J Immunol 2009 Dec 15;183(12):7975-83 (Medline PMID: 19923465)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030343-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030343-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030343-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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