Strain Detail Sheet

Strain Name    :

C57BL/6J-Cd3em1Btlr/Mmucd

Stock Number :

030345-UCD

Other Names   :

tumormouse, C57BL/6J-Cd3em1Btlr/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Cd3em1Btlr
Name: mutation 1, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Cd3e
Name: CD3 antigen, epsilon polypeptide
Chromosome: 9
Alteration at locus: Chemically Induced

Genetic Alterations:
ENU-induced C to A transition at position 330 of the Cd3e gene (Genbank Accession NM_007648); replaces the codon for tyrosine 84 with a premature stop codon. The early truncation results in a CD3epsilon null animal.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: The tumormouse phenotype was identified in a screen for allogeneic sterility. The index mutant was injected intraperitoneally with L-929 fibroblasts of C3H background in order to pre-immunize against C3H alloreactive determinants. The animal developed an intra-abdominal tumor consisting of L-929 cells, hence the designation tumormouse.

Homozygous tumormouse animals have very small thymi with abnormal structure and no peripheral T cells. Tumormouse mutants have no CD4+CD8+ double positive (DP) T cells, and thymocyte development is arrested at the CD44+CD25- double negative (DN) 3 stage. Compared to wild type, tumormouse thymocytes display increased levels of apoptosis. B cells, macrophages and NK cells are found at normal levels in the periphery in tumormouse animals.

Some homozygous tumormouse animals older than 9 months of age appear to experience epileptic-like seizures.

Heterozygous phenotype: wild type


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Cd3em1Btlr/Cd3em1Btlr
        C57BL/6J-Cd3e<m1Btlr>
  • hematopoietic system phenotype
    • abnormal thymus morphology (MGI Ref ID J:132486)
      • thymi of homozygous mutant mice are structurally abnormal
      • small thymus (MGI Ref ID J:132486)
        • homozygous mutant mice have very small thymi
    • absent T cells (MGI Ref ID J:132486)
      • homozygous mutants lack peripheral T lymphocytes
      • no CD4+CD8+ T cells are present in mutant thymi
    • arrested T cell differentiation (MGI Ref ID J:132486)
      • development of mutant thymocytes is arrested at the CD44+CD25-, double negative 3 (DN3) stage
  • immune system phenotype
    • abnormal thymus morphology (MGI Ref ID J:132486)
      • thymi of homozygous mutant mice are structurally abnormal
      • small thymus (MGI Ref ID J:132486)
        • homozygous mutant mice have very small thymi
    • absent T cells (MGI Ref ID J:132486)
      • homozygous mutants lack peripheral T lymphocytes
      • no CD4+CD8+ T cells are present in mutant thymi
    • arrested T cell differentiation (MGI Ref ID J:132486)
      • development of mutant thymocytes is arrested at the CD44+CD25-, double negative 3 (DN3) stage
    • increased T cell apoptosis (MGI Ref ID J:132486)
      • homozygous mutant thymocytes undergo apoptosis at a higher rate than do those of wild-type mice
  • behavior/neurological phenotype
    • seizures (MGI Ref ID J:132486)
      • some homozygous mutants older than 9 months appear to experience epileptic-like seizures
  • nervous system phenotype
    • seizures (MGI Ref ID J:132486)
      • some homozygous mutants older than 9 months appear to experience epileptic-like seizures

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: Original mutant is a C57BL/6J G3 ENU- induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cell Biology
  • Developmental Biology
  • Immunology and Inflammation
  • Research Tools

Strain Origin

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

  • Berger M; Krebs P; Crozat K; Li X; Croker BA; Siggs OM; Popkin D; Du X; Lawson BR; Theofilopoulos AN; Xia Y; Khovananth K; Y Moresco EM; Satoh T; Takeuchi O; Akira S; Beutler B, An Slfn2 mutation causes lymphoid and myeloid immunodeficiency due to loss of immune cell quiescence., Nat Immunol 2010 Apr;11(4):335-43 (Medline PMID: 20190759)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030345-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030345-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
030345-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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