Strain Detail Sheet

Strain Name    :

B6.129X1-Robo3tm1Matl/Mmmh

Stock Number :

030402-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Robo3tm1Matl
Name: targeted mutation 1, Marc Tessier-Lavigne
Alteration at locus: Targeted Mutation
Gene Symbol: Robo3
Name: roundabout homolog 3 (Drosophila)
Chromosome: 9
Alteration at locus: Targeted Mutation

Genetic Alterations:
IRES-TauGFP- loxpNeomycin insertion into exon1

Genotype Determination:

ES Cell Line: Not specified derived from B6;129X1

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Perinatal lethality. Failure of commissural axons to cross midline

Heterozygous phenotype: None


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Robo3tm1Matl/Robo3tm1Matl
        involves: C57BL/6
  • mortality/aging
    • complete neonatal lethality (MGI Ref ID J:165285)
      • within 8 hours of birth
  • nervous system phenotype
    • abnormal pre-Botzinger complex physiology (MGI Ref ID J:165285)
      • at E15.5, rhythmic activity frequency in the pre-Botzinger complex is lower than in wild-type mice
      • at E15.5, bilateral synchronization of rhythmic activity in the pre-Botzinger complex is absent unlike in wild-type mice
  • respiratory system phenotype
    • abnormal breathing pattern (MGI Ref ID J:165285)
      • at P0, mice exhibit independent left and right rhythmic contractions of the diaphragm unlike wild-type mice
  • homeostasis/metabolism phenotype


The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Robo3tm1Matl/Robo3tm1Matl
        Background Not Specified
  • nervous system phenotype
    • abnormal brain commissure morphology (MGI Ref ID J:158918)
      • at E14 and in newborns, virtually no axons are found to cross the midline at the level of the abducens nuclei
Robo3tm1Matl/Robo3tm1Matl
        either: (involves: 129/Sv * C57BL/6) or (involves: 129/Sv * CD-1)
  • mortality/aging
    • complete perinatal lethality (MGI Ref ID J:89438)
      • homozygous null mice are born alive but die within a few hours
  • nervous system phenotype
    • abnormal axon guidance (MGI Ref ID J:89438)
      • from E11.5 - E14.5 at all axial levels of the spinal cord and hindbrain commissural axons fail to cross the floor plate and instead veer away after reaching the vicinity of the floor plate
      • after failing to cross the floor plate the commissural axons grow longitudinally along the ipsilateral side of the floor plate in a disorganized manner with most failing to stay close to the floor plate, some stalled close to the floor plate, and others turning rostrally, caudally, or bifurcating and growing in both directions
      • in vitro assays indicate axons from homozygous mutants are prematurely Slit responsive
    • abnormal floor plate morphology (MGI Ref ID J:89438)
      • the spinal cord fails to stay attached at the ventral midline during dissection indicating a thin or fragile floor plate
  • embryogenesis phenotype
    • abnormal floor plate morphology (MGI Ref ID J:89438)
      • the spinal cord fails to stay attached at the ventral midline during dissection indicating a thin or fragile floor plate

Strain of Origin: 129X1/SvJ

Strain genetic background: C57BL/6N

Suggested Control Mice: Wild-type littermates

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor: Marc Tessier-Lavigne, Ph.D., Genentech

Primary Reference:

Sabatier C, Plump AS, Le Ma, Brose K, Tamada A, Murakami F, Lee EY, Tessier-Lavigne M. The divergent Robo family protein rig-1/Robo3 is a negative regulator of slit responsiveness required for midline crossing by commissural axons. Cell. 2004 Apr 16;117(2):157-69. (Medline PMID: 15084255)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
030402-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
030402-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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